Facilitation of hypothermia by quinpirole and 8-OH-DPAT in a rat model of cardiac arrest
Aim of the study: Therapeutic hypothermia improves outcome after cardiac arrest. Dopamine D2 agonists and serotonin 5-HT1A agonists lower body temperature by decreasing the set-point. We investigated the effect of these drugs on temperature and cerebral recovery of rats after cardiac arrest. Methods...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2012
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| In: |
Resuscitation
Year: 2011, Volume: 83, Issue: 2, Pages: 232-237 |
| ISSN: | 1873-1570 |
| DOI: | 10.1016/j.resuscitation.2011.07.023 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.resuscitation.2011.07.023 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0300957211004606 
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| Author Notes: | Andreas Schneider, Peter Teschendorf, Peter Vogel, Nicolai Russ, Jürgen Knapp, Bernd W. Böttiger, Erik Popp |
| Summary: | Aim of the study: Therapeutic hypothermia improves outcome after cardiac arrest. Dopamine D2 agonists and serotonin 5-HT1A agonists lower body temperature by decreasing the set-point. We investigated the effect of these drugs on temperature and cerebral recovery of rats after cardiac arrest. Methods: Male Wistar-Han rats were subjected to 6min of cardiac arrest due to ventricular fibrillation. Following restoration of circulation, 1mg quinpirole, 1mg 8-OH-DPAT or vehicle were injected subcutaneously. Body temperature was monitored for 48h. One additional group was kept normothermic. Animals were neurologically tested by a tape removal test. After 7 days, histology of hippocampal CA-1 sector was analysed with Nissl and TUNEL staining. Results: Rats became spontaneously hypothermic after cardiac arrest. Induction of hypothermia was facilitated by both quinpirole (−0.033±0.008°C/min) and 8-OH-DPAT (−0.029±0.010°C/min) when compared to vehicle (−0.020±0.005°C/min). Total ‘dose’ of hypothermia (area under the curve) was not different. All animals showed a neurological deficit, which improved with time; after 7 days, test results of the normothermic group (30 [11-88]s) still tended to be worse than those of the hypothermic groups (vehicle 8 [6-14]s, quinpirole 9 [4-17]s, 8-OH-DPAT 10 [8-22]s). There were no clear differences in Nissl or TUNEL histology after 7 days. Conclusion: Both quinpirole and 8-OH-DPAT led to faster induction of hypothermia. However, the outcome was not different from spontaneous hypothermia, probably because the total ‘dose’ of hypothermia was not influenced. |
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| Item Description: | Available online 29 July 2011 Gesehen am 25.06.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1873-1570 |
| DOI: | 10.1016/j.resuscitation.2011.07.023 |