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Neuronal LRP4 regulates synapse formation in the developing CNS

Skip to Next Section The low-density lipoprotein receptor-related protein 4 (LRP4) is essential in muscle fibers for the establishment of the neuromuscular junction. Here, we show that LRP4 is also expressed by embryonic cortical and hippocampal neurons, and that downregulation of LRP4 in these neur...

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Bibliographic Details
Main Authors: Karakatsani, Andromachi (Author) , Urban, Severino (Author) , Ruiz de Almodóvar, Carmen (Author)
Format: Article (Journal)
Language:English
Published: 2017
In: Development
Year: 2017, Volume: 144, Issue: 24, Pages: 4604-4615
ISSN:1477-9129
DOI:10.1242/dev.150110
Online Access:Verlag, Volltext: http://dx.doi.org/10.1242/dev.150110
Verlag, Volltext: http://dev.biologists.org/content/144/24/4604
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Author Notes:Andromachi Karakatsani, Nicolás Marichal, Severino Urban, Georgios Kalamakis, Alexander Ghanem, Anna Schick, Yina Zhang, Karl-Klaus Conzelmann, Markus A. Rüegg, Benedikt Berninger, Carmen Ruiz de Almodovar, Sergio Gascón, Stephan Kröger
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Summary:Skip to Next Section The low-density lipoprotein receptor-related protein 4 (LRP4) is essential in muscle fibers for the establishment of the neuromuscular junction. Here, we show that LRP4 is also expressed by embryonic cortical and hippocampal neurons, and that downregulation of LRP4 in these neurons causes a reduction in density of synapses and number of primary dendrites. Accordingly, overexpression of LRP4 in cultured neurons had the opposite effect inducing more but shorter primary dendrites with an increased number of spines. Transsynaptic tracing mediated by rabies virus revealed a reduced number of neurons presynaptic to the cortical neurons in which LRP4 was knocked down. Moreover, neuron-specific knockdown of LRP4 by in utero electroporation of LRP4 miRNA in vivo also resulted in neurons with fewer primary dendrites and a lower density of spines in the developing cortex and hippocampus. Collectively, our results demonstrate an essential and novel role of neuronal LRP4 in dendritic development and synaptogenesis in the CNS.
Item Description:Published online December 18, 2017
Gesehen am 25.06.2018
Physical Description:Online Resource
ISSN:1477-9129
DOI:10.1242/dev.150110

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