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Pathogenic role of the damage-associated molecular patterns S100A8 and S100A9 in coxsackievirus B3-induced myocarditis

Background: The alarmins S100A8 and S100A9 are damage-associated molecular patterns, which play a pivotal role in cardiovascular diseases, inflammation, and viral infections. We aimed to investigate their role in Coxsackievirus B3 (CVB3)-induced myocarditis. Methods and Results: S100A8 and S100A9 mR...

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Main Authors: Müller, Irene (Author) , Rohde, David (Author) , Most, Patrick (Author)
Format: Article (Journal)
Language:English
Published: November 20, 2017
In: Circulation. Heart failure
Year: 2017, Volume: 10, Issue: 11
ISSN:1941-3297
DOI:10.1161/CIRCHEARTFAILURE.117.004125
Online Access:Verlag, teilw. kostenfrei, Volltext: http://dx.doi.org/10.1161/CIRCHEARTFAILURE.117.004125
Verlag, teilw. kostenfrei, Volltext: http://circheartfailure.ahajournals.org.ezproxy.medma.uni-heidelberg.de/content/10/11/e004125
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Author Notes:Irene Müller, Thomas Vogl, Kathleen Pappritz, Kapka Miteva, Konstantinos Savvatis, David Rohde, Patrick Most, Dirk Lassner, Burkert Pieske, Uwe Kühl, Sophie Van Linthout, Carsten Tschöpe
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Summary:Background: The alarmins S100A8 and S100A9 are damage-associated molecular patterns, which play a pivotal role in cardiovascular diseases, inflammation, and viral infections. We aimed to investigate their role in Coxsackievirus B3 (CVB3)-induced myocarditis. Methods and Results: S100A8 and S100A9 mRNA expression was 13.0-fold (P=0.012) and 5.1-fold (P=0.038) higher in endomyocardial biopsies from patients with CVB3-positive myocarditis compared with controls, respectively. Elimination of CVB3 led to a downregulation of these alarmins. CVB3-infected mice developed an impaired left ventricular function and displayed an increased left ventricular S100A8 and S100A9 protein expression versus controls. In contrast, CVB3-infected S100A9 knockout mice, which are also a complete knockout for S100A8 on protein level, showed an improved left ventricular function, which was associated with a reduced cardiac inflammatory and oxidative response, and lower CVB3 copy number compared with wild-type CVB3 mice. Exogenous application of S100A8 to S100A9 knockout CVB3 mice induced a severe myocarditis similar to wild-type CVB3 mice. In CVB3-infected HL-1 cells, S100A8 and S100A9 enhanced oxidative stress and CVB3 copy number compared with unstimulated infected cells. In CVB3-infected RAW macrophages, both alarmins increased MIP-2 (macrophage inflammatory protein-2) chemokine expression, which was reduced in CVB3 S100A8 knockdown versus scrambled siRNA CVB3 cells. Conclusions: S100A8 and S100A9 aggravate CVB3-induced myocarditis and might serve as therapeutic targets in inflammatory cardiomyopathies.
Item Description:Gesehen am 04.07.2018
Physical Description:Online Resource
ISSN:1941-3297
DOI:10.1161/CIRCHEARTFAILURE.117.004125

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