7-Aminoactinomycin D for apoptosis staining in flow cytometry
7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. Several staining protocols using 7-AAD have been described, but data on the influence of the 7-AAD concentration on the readout are not available. Therefore, we compared t...
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| Main Authors: | , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
14 July 2012
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| In: |
Analytical biochemistry
Year: 2012, Volume: 429, Issue: 1, Pages: 79-81 |
| ISSN: | 1096-0309 |
| DOI: | 10.1016/j.ab.2012.07.005 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.ab.2012.07.005 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0003269712003569 
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| Author Notes: | Nadine C.L. Zembruski, Vanessa Stache, Walter E. Haefeli, Johanna Weiss |
| Summary: | 7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. Several staining protocols using 7-AAD have been described, but data on the influence of the 7-AAD concentration on the readout are not available. Therefore, we compared the results obtained by staining with 1, 5, 10, and 20μg/ml 7-AAD for 20min with the PE-Annexin V Apoptosis Detection Kit and Cell Death Detection ELISAPLUS in lymphocytes and CEM human leukemia cells. The results showed that 7-AAD staining with 5, 10, and 20μg/ml, but not with 1μg/ml, is suitable for quantification of apoptosis in flow cytometry. |
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| Item Description: | Gesehen am 21.07.2020 |
| Physical Description: | Online Resource |
| ISSN: | 1096-0309 |
| DOI: | 10.1016/j.ab.2012.07.005 |