Macrophages discriminate glycosylation patterns of apoptotic cell-derived microparticles
Inappropriate clearance of apoptotic remnants is considered to be the primary cause of systemic autoimmune diseases, like systemic lupus erythematosus. Here we demonstrate that apoptotic cells release distinct types of subcellular membranous particles (scMP) derived from the endoplasmic reticulum (E...
Gespeichert in:
| Hauptverfasser: | , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
2012
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| In: |
The journal of biological chemistry
Year: 2011, Jahrgang: 287, Heft: 1, Pages: 496-503 |
| ISSN: | 1083-351X |
| DOI: | 10.1074/jbc.M111.273144 |
| Online-Zugang: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1074/jbc.M111.273144 Verlag, kostenfrei, Volltext: http://www.jbc.org/content/287/1/496 
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| Verfasserangaben: | Rostyslav O. Bilyy, Tanya Shkandina, Andriy Tomin, Luis E. Muñoz, Sandra Franz, Volodymyr Antonyuk, Yuriy Ya Kit, Matthias Zirngibl, Barbara G. Fürnrohr, Christina Janko, Kirsten Lauber, Martin Schiller, Georg Schett, Rostyslav S. Stoika, and Martin Herrmann |
| Zusammenfassung: | Inappropriate clearance of apoptotic remnants is considered to be the primary cause of systemic autoimmune diseases, like systemic lupus erythematosus. Here we demonstrate that apoptotic cells release distinct types of subcellular membranous particles (scMP) derived from the endoplasmic reticulum (ER) or the plasma membrane. Both types of scMP exhibit desialylated glycotopes resulting from surface exposure of immature ER-derived glycoproteins or from surface-borne sialidase activity, respectively. Sialidase activity is activated by caspase-dependent mechanisms during apoptosis. Cleavage of sialidase Neu1 by caspase 3 was shown to be directly involved in apoptosis-related increase of surface sialidase activity. ER-derived blebs possess immature mannosidic glycoepitopes and are prioritized by macrophages during clearance. Plasma membrane-derived blebs contain nuclear chromatin (DNA and histones) but not components of the nuclear envelope. Existence of two immunologically distinct types of apoptotic blebs may provide new insights into clearance-related diseases. |
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| Beschreibung: | Gesehen am 06.08.2018 Received for publication, June 17, 2011, and in revised form, November 2, 2011, published November 10, 2011 |
| Beschreibung: | Online Resource |
| ISSN: | 1083-351X |
| DOI: | 10.1074/jbc.M111.273144 |