Effects of ethanol on the properties of platelets and endothelial cells in model experiments
Aim: To investigate effects of ethanol on activity markers of atherosclerosis in an in vitro endothelial cell model. Methods: After 24 h incubation with ethanol (0.0095%), human umbilical vein endothelial cells were stimulated for 1 h with lipopolysaccharide, and were then incubated in direct contac...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
June 26, 2012
|
| In: |
World journal of cardiology
Year: 2012, Volume: 4, Issue: 6, Pages: 201-205 |
| ISSN: | 1949-8462 |
| DOI: | 10.4330/wjc.v4.i6.201 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.4330/wjc.v4.i6.201 Verlag, Volltext: https://www.wjgnet.com/1949-8462/full/v4/i6/201.htm 
|
| Author Notes: | Ksenija Stach, Anna-Isabelle Kälsch, Christel Weiß, Elif Elmas, Martin Borggrefe, Thorsten Kälsch |
| Summary: | Aim: To investigate effects of ethanol on activity markers of atherosclerosis in an in vitro endothelial cell model. Methods: After 24 h incubation with ethanol (0.0095%), human umbilical vein endothelial cells were stimulated for 1 h with lipopolysaccharide, and were then incubated in direct contact with activated platelets. Following this incubation, the expression of CD40L and CD62P on platelets, and the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), urokinase plasminogen activator receptor (uPAR), and membrane-type 1 matrix metalloproteinase (MT1-MMP) on endothelial cells were measured by flow cytometry. Results: The increased expression of VCAM-1 and uPAR on endothelial cells by proinflammatory stimulation with activated platelets was significantly reduced through pre-incubation with ethanol (P < 0.05). Furthermore, platelets in direct contact with ethanol and with endothelial cells pre-incubated in ethanol showed a significant reduction in their CD40L expression (P < 0.05). Ethanol had no significant effect on ICAM-1 and MT1-MMP expression on endothelial cells. Conclusion: Ethanol directly attenuates platelet activation and has significant endothelial cell-mediated effects on selected markers of atherosclerosis in vitro. These findings underline possible protective effects of ethanol on atherosclerosis. |
|---|---|
| Item Description: | Published online: June 26, 2012 Gesehen am 09.08.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1949-8462 |
| DOI: | 10.4330/wjc.v4.i6.201 |