Protocol for efficient generation and characterization of adeno-associated viral vectors
Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct compa...
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| Main Authors: | , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
1 Oct 2017
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| In: |
Human gene therapy. Methods
Year: 2017, Volume: 28, Issue: 5, Pages: 235-246 |
| ISSN: | 1946-6544 |
| DOI: | 10.1089/hgtb.2017.192 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1089/hgtb.2017.192 Verlag, Volltext: https://www.liebertpub.com/doi/10.1089/hgtb.2017.192 
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| Author Notes: | Andreas Jungmann, Barbara Leuchs, Jean Rommelaere, Hugo A. Katus, and Oliver J. Müller |
| Summary: | Adeno-associated virus vectors are a powerful tool for gene transfer approaches. We have established a simple and fast plasmid-based production system for achieving high adeno-associated virus titers within 6 working days. The same procedure can be used for all serotypes and thus allows direct comparability of different serotypes. In this protocol we describe a step-by-step procedure that results in well-characterized vectors suitable for both in vitro approaches and preclinical studies. |
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| Item Description: | Gesehen am 10.08.2018 Published Online:1 October 2017 |
| Physical Description: | Online Resource |
| ISSN: | 1946-6544 |
| DOI: | 10.1089/hgtb.2017.192 |