Super-resolved linear fluorescence localization microscopy using photostable fluorophores: a virtual microscopy study
Current approaches to overcome the conventional limit of the resolution potential of light microscopy (of about 200 nm for visible light), often suffer from non-linear effects, which render the quantification of the image intensities in the reconstructions difficult, and also affect the quantificati...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
21 July 2017
|
| In: |
Optics communications
Year: 2017, Volume: 404, Pages: 42-50 |
| ISSN: | 1873-0310 |
| DOI: | 10.1016/j.optcom.2017.06.078 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.optcom.2017.06.078 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0030401817305540 
|
| Author Notes: | Udo Birk, Aleksander Szczurek, Christoph Cremer |
| Summary: | Current approaches to overcome the conventional limit of the resolution potential of light microscopy (of about 200 nm for visible light), often suffer from non-linear effects, which render the quantification of the image intensities in the reconstructions difficult, and also affect the quantification of the biological structure under investigation. As an attempt to face these difficulties, we discuss a particular method of localization microscopy which is based on photostable fluorescent dyes. The proposed method can potentially be implemented as a fast alternative for quantitative localization microscopy, circumventing the need for the acquisition of thousands of image frames and complex, highly dye-specific imaging buffers. Although the need for calibration remains in order to extract quantitative data (such as the number of emitters), multispectral approaches are largely facilitated due to the much less stringent requirements on imaging buffers. Furthermore, multispectral acquisitions can be readily obtained using commercial instrumentation such as e.g. the conventional confocal laser scanning microscope. |
|---|---|
| Item Description: | Available online 21 July 2017 Gesehen am 14.08.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1873-0310 |
| DOI: | 10.1016/j.optcom.2017.06.078 |