Riboflavin carrier protein-targeted fluorescent USPIO for the assessment of vascular metabolism in tumors
Riboflavin (Rf) and its metabolic analogs flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are essential for normal cellular growth and function. Their intracellular transport is regulated by the riboflavin carrier protein (RCP), which has been shown to be over-expressed by metaboli...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
6 September 2012
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| In: |
Biomaterials
Year: 2012, Volume: 33, Issue: 34, Pages: 8822-8829 |
| ISSN: | 1878-5905 |
| DOI: | 10.1016/j.biomaterials.2012.08.036 |
| Online Access: | Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0142961212009398 Verlag, Volltext: http://dx.doi.org/10.1016/j.biomaterials.2012.08.036 
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| Author Notes: | Jabadurai Jayapaul, Susanne Arns, Wiltrud Lederle, Twan Lammers, Peter Comba, Jessica Gätjens, Fabian Kiessling |
| Summary: | Riboflavin (Rf) and its metabolic analogs flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are essential for normal cellular growth and function. Their intracellular transport is regulated by the riboflavin carrier protein (RCP), which has been shown to be over-expressed by metabolically active cancer cells. Therefore, FAD-decorated ultrasmall superparamagnetic iron oxide nanoparticles (FAD USPIO) were developed as the first carrier-protein-targeted molecular MR agents for visualizing tumor metabolism. FAD USPIO were synthesized using an adsorptive, fluorescent and non-polymeric coating method, and their physicochemical properties were characterized using TEM, SEM, FTIR, MRI and fluorescence spectroscopy. In vitro analyses showed the biocompatibility of FAD USPIO, and confirmed that they were strongly and specifically taken up by cancer (LnCap) and endothelial (HUVEC) cells. In vivo molecular MRI together with subsequent histological validation finally demonstrated that FAD USPIO efficiently accumulate in tumors and tumor blood vessels, indicating that RCP-targeted diagnostic nanoparticles are interesting new materials for the assessment of vascular metabolism in tumors. |
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| Item Description: | Available online 6 September 2012 Gesehen am 16.08.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1878-5905 |
| DOI: | 10.1016/j.biomaterials.2012.08.036 |