Haemoglobin S and C affect the motion of Maurer's clefts in Plasmodium falciparum-infected erythrocytes
The haemoglobinopathies S and C protect carriers from severe Plasmodium falciparum malaria. We have recently shown that haemoglobin S and C interfere with host-actin remodelling in parasitized erythrocytes and the generation of an actin network that seems to be required for vesicular protein traffic...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
2013
|
| In: |
Cellular microbiology
Year: 2013, Volume: 15, Issue: 7, Pages: 1111-1126 |
| ISSN: | 1462-5822 |
| DOI: | 10.1111/cmi.12102 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1111/cmi.12102 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1111/cmi.12102 
|
| Author Notes: | Nicole Kilian, Martin Dittmer, Marek Cyrklaff, Djeneba Ouermi, Cyrille Bisseye, Jacques Simpore, Friedrich Frischknecht, Cecilia P. Sanchez, Michael Lanzer |
| Summary: | The haemoglobinopathies S and C protect carriers from severe Plasmodium falciparum malaria. We have recently shown that haemoglobin S and C interfere with host-actin remodelling in parasitized erythrocytes and the generation of an actin network that seems to be required for vesicular protein trafficking from the Maurer's clefts (a parasite-derived intermediary protein secretory organelle) to the erythrocyte surface. Here we show that the actin network exerts skeletal functions by anchoring the Maurer's clefts within the erythrocyte cytoplasm. Using a customized tracking tool to investigate the motion of single Maurer's clefts, we found that a functional actin network restrains Brownian motion of this organelle. Maurer's clefts moved significantly faster in wild-type erythrocytes treated with the actin depolymerizing agent cytochalasin D and in erythrocytes containing the haemoglobin variants S and C. Our data support the model of an impaired actin network being an underpinning cause of cellular malfunctioning in parasitized erythrocytes containing haemoglobin S or C, and, possibly, for the protective role of these haemoglobin variants against severe malaria. |
|---|---|
| Item Description: | First published online 20 January 2013 Gesehen am 23.08.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1462-5822 |
| DOI: | 10.1111/cmi.12102 |