Altered plasma apolipoprotein modifications in patients with pancreatic cancer: protein characterization and multi-institutional validation
Background: Among the more common human malignancies, invasive ductal carcinoma of the pancreas has the worst prognosis. The poor outcome seems to be attributable to difficulty in early detection. Methods: We compared the plasma protein profiles of 112 pancreatic cancer patients with those of 103 se...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
October 8, 2012
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| In: |
PLOS ONE
Year: 2012, Volume: 7, Issue: 10 |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0046908 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1371/journal.pone.0046908 Verlag, kostenfrei, Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0046908 
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| Author Notes: | Kazufumi Honda, Takuji Okusaka, Klaus Felix, Shoji Nakamori, Naohiro Sata, Hideo Nagai, Tatsuya Ioka, Akihiko Tsuchida, Takeshi Shimahara, Masashi Shimahara, Yohichi Yasunami, Hideya Kuwabara, Tomohiro Sakuma, Yoshihiko Otsuka, Norihito Ota, Miki Shitashige, Tomoo Kosuge, Markus W. Büchler, Tesshi Yamada |
| Summary: | Background: Among the more common human malignancies, invasive ductal carcinoma of the pancreas has the worst prognosis. The poor outcome seems to be attributable to difficulty in early detection. Methods: We compared the plasma protein profiles of 112 pancreatic cancer patients with those of 103 sex- and age-matched healthy controls (Cohort 1) using a newly developed matrix-assisted laser desorption/ionization (oMALDI) QqTOF (quadrupole time-of-flight) mass spectrometry (MS) system. Results: We found that hemi-truncated apolipoprotein AII dimer (ApoAII-2; 17252 m/z), unglycosylated apolipoprotein CIII (ApoCIII-0; 8766 m/z), and their summed value were significantly decreased in the pancreatic cancer patients [P = 1.36×10−21, P = 4.35×10−14, and P = 1.83×10−24 (Mann-Whitney U-test); area-under-curve values of 0.877, 0.798, and 0.903, respectively]. The significance was further validated in a total of 1099 plasma/serum samples, consisting of 2 retrospective cohorts [Cohort 2 (n = 103) and Cohort 3 (n = 163)] and a prospective cohort [Cohort 4 (n = 833)] collected from 8 medical institutions in Japan and Germany. Conclusions: We have constructed a robust quantitative MS profiling system and used it to validate alterations of modified apolipoproteins in multiple cohorts of patients with pancreatic cancer. |
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| Item Description: | Gesehen am 29.08.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0046908 |