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Reverse-transcription loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus directly from nasopharyngeal swabs

Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract infection in young infants and a major cause of nosocomial infection in pediatric care. Currently available RSV point-of-care tests are of limited sensitivity and relatively expensive. We developed and evaluated a novel R...

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Main Authors: Hoos, Johannes (Author) , Peters, Rebecca (Author) , Tabatabai, Julia (Author) , Grulich-Henn, Jürgen (Author) , Schnitzler, Paul (Author) , Pfeil, Johannes (Author)
Format: Article (Journal)
Language:English
Published: 16 January 2017
In: Journal of virological methods
Year: 2017, Volume: 242, Pages: 53-57
ISSN:1879-0984
DOI:10.1016/j.jviromet.2017.01.006
Online Access:Verlag, Volltext: http://dx.doi.org/10.1016/j.jviromet.2017.01.006
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0166093416307261
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Author Notes:Johannes Hoos, Rebecca Marie Peters, Julia Tabatabai, Jürgen Grulich-Henn, Paul Schnitzler, Johannes Pfeil
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Summary:Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract infection in young infants and a major cause of nosocomial infection in pediatric care. Currently available RSV point-of-care tests are of limited sensitivity and relatively expensive. We developed and evaluated a novel RSV rapid test for use at point-of-care, based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) for direct testing of nasopharyngeal swab specimens. RT-LAMP can detect RSV within 30min, without the need for RNA extraction. The sensitivity of our RT-LAMP assay was 70-80% in comparison to RT-PCR. The RT-LAMP test sensitivity is at least equivalent to currently available rapid antigen detection tests (RADT), and the cost of RT-LAMP test reagents is only approximately 10% of that of commercially available RADT tests. RT-LAMP appears to be an attractive alternative to RADT, particularly in settings with limited financial resources. Future improvements could include lyophilization of test reagents and automated read-out of RT-LAMP results.
Item Description:Gesehen am 30.08.2018
Physical Description:Online Resource
ISSN:1879-0984
DOI:10.1016/j.jviromet.2017.01.006

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