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Chd7 is indispensable for mammalian brain development through activation of a neuronal differentiation programme

Mutations in chromatin modifier genes are frequently associated with neurodevelopmental diseases. We herein demonstrate that the chromodomain helicase DNA-binding protein 7 (Chd7), frequently associated with CHARGE syndrome, is indispensable for normal cerebellar development. Genetic inactivation of...

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Main Authors: Feng, Weijun (Author) , Kawauchi, Daisuke (Author) , Hanna, Bola (Author) , Korshunov, Andrey (Author) , Jones, David T. W. (Author) , Kool, Marcel (Author) , Lichter, Peter (Author) , Pfister, Stefan (Author) , Liu, Hai-Kun (Author)
Format: Article (Journal)
Language:English
Published: 20 Mar 2017
In: Nature Communications
Year: 2017, Volume: 8, Pages: 14758
ISSN:2041-1723
DOI:10.1038/ncomms14758
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1038/ncomms14758
Verlag, kostenfrei, Volltext: https://www.nature.com/articles/ncomms14758
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Author Notes:Weijun Feng, Daisuke Kawauchi, Huiqin Körkel-Qu, Huan Deng, Elisabeth Serger, Laura Sieber, Jenna Ariel Lieberman, Silvia Jimeno-González, Sander Lambo, Bola S. Hanna, Yassin Harim, Malin Jansen, Anna Neuerburg, Olga Friesen, Marc Zuckermann, Vijayanad Rajendran, Jan Gronych, Olivier Ayrault, Andrey Korshunov, David T.W. Jones, Marcel Kool, Paul A. Northcott, Peter Lichter, Felipe Cortés-Ledesma, Stefan M. Pfister & Hai-Kun Liu
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Summary:Mutations in chromatin modifier genes are frequently associated with neurodevelopmental diseases. We herein demonstrate that the chromodomain helicase DNA-binding protein 7 (Chd7), frequently associated with CHARGE syndrome, is indispensable for normal cerebellar development. Genetic inactivation of Chd7 in cerebellar granule neuron progenitors leads to cerebellar hypoplasia in mice, due to the impairment of granule neuron differentiation, induction of apoptosis and abnormal localization of Purkinje cells, which closely recapitulates known clinical features in the cerebella of CHARGE patients. Combinatory molecular analyses reveal that Chd7 is required for the maintenance of open chromatin and thus activation of genes essential for granule neuron differentiation. We further demonstrate that both Chd7 and Top2b are necessary for the transcription of a set of long neuronal genes in cerebellar granule neurons. Altogether, our comprehensive analyses reveal a mechanism with chromatin remodellers governing brain development via controlling a core transcriptional programme for cell-specific differentiation.
Item Description:Gesehen am 30.08.2019
Physical Description:Online Resource
ISSN:2041-1723
DOI:10.1038/ncomms14758

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