Monocyte-dependent suppression of T-cell function in postoperative patients and abdominal sepsis
Introduction: Surgical trauma causes inflammation and postoperative immunosuppression. Previous studies have shown a T-cell-dependent suppression of MHC II expression and other functions of antigen-presenting cells. The aim of this study was to determine which immune cell initiates postoperative imm...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
December 2017
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| In: |
Shock
Year: 2017, Volume: 48, Issue: 6, Pages: 651-656 |
| ISSN: | 1540-0514 |
| DOI: | 10.1097/SHK.0000000000000924 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1097/SHK.0000000000000924 Verlag, Volltext: http://journals.lww.com/shockjournal/fulltext/2017/12000/Monocyte_Dependent_Suppression_of_T_Cell_Function.8.aspx 
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| Author Notes: | Markus Albertsmeier, Niclas J. Prix, Hauke Winter, Alexandr Bazhin, Jens Werner, and Martin K. Angele |
| Summary: | Introduction: Surgical trauma causes inflammation and postoperative immunosuppression. Previous studies have shown a T-cell-dependent suppression of MHC II expression and other functions of antigen-presenting cells. The aim of this study was to determine which immune cell initiates postoperative immunosuppression and consecutive sepsis. Methods: We separated T-cells and monocytes in human abdominal surgery (n = 11) patients preoperatively as well as 24 h postoperatively and in patients who developed postoperative sepsis (n = 6). We analyzed their surface markers and then coincubated these cells with naïve preoperative cells of the other cell type, respectively. Cytokine secretion from naïve cells was measured by a multiplex immunoassay, serving as a bioassay for the function of the stimulating postoperative cell. Results: Surface marker analysis showed a postoperative suppression of CD3+ cells and the activation marker CD28 (P = 0.02), which was further reduced in septic patients. FACS analysis revealed a significant increase in CD14+ monocytes (P = 0.02) and CD14+CD86+, CD14+HLA-DR+ subpopulations 2 h postoperatively. In sepsis patients, HLA-DR expression was reduced compared with postoperative levels (P < 0.01). After coincubation with postoperative T-cells, secretion of IL-6 (P < 0.01) and IL-10 (P < 0.01) from naïve monocytes was increased, whereas T-cells from sepsis patients resulted in suppressed cytokine secretion. After coincubation with postoperative monocytes, secretion of IFN-gamma (P < 0.01) and IL-10 (P < 0.01) from naïve T-cells was significantly diminished, whereas monocytes from septic patients triggered only insignificant IL-10 secretion from naïve and septic T-cells. Conclusions: Our results show that in the early postoperative period, T-cells are suppressed but able to trigger the release of cytokines from monocytes, whereas activated monocytes seem to induce T-cell suppression. In sepsis patients, a global suppression of both cell types in terms of absolute numbers and function seems to occur. |
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| Item Description: | Gesehen am 13.09.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1540-0514 |
| DOI: | 10.1097/SHK.0000000000000924 |