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In vivo protein crystallization opens new routes in structural biology

Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of fre...

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Main Authors: Koopmann, Rudolf (Author) , Epp, Sascha W. (Author) , Erk, Benjamin Michael Dieter (Author) , Foucar, Lutz Michael (Author) , Kassemeyer, Stephan (Author) , Lomb, Lukas (Author) , Rolles, Daniel (Author) , Rudek, Benedikt (Author) , Rudenko, Artem (Author) , Schlichting, Ilme (Author) , Shoeman, Robert L. (Author) , Ullrich, Joachim (Author)
Format: Article (Journal)
Language:English
Published: 2012 Jan 29
In: Nature methods
Year: 2012, Volume: 9, Issue: 3, Pages: 259-262
ISSN:1548-7105
DOI:10.1038/nmeth.1859
Online Access:Verlag, Volltext: http://dx.doi.org/10.1038/nmeth.1859
Verlag, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429599/
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Author Notes:Rudolf Koopmann, Karolina Cupelli, Lars Redecke, Karol Nass, Daniel P DePonte, Thomas A White, Francesco Stellato, Dirk Rehders, Mengning Liang, Jakob Andreasson, Andrew Aquila, Sasa Bajt, Miriam Barthelmess, Anton Barty, Michael J Bogan, Christoph Bostedt, Sébastien Boutet, John D Bozek, Carl Caleman, Nicola Coppola, Jan Davidsson, R Bruce Doak, Tomas Ekeberg, Sascha W Epp, Benjamin Erk, Holger Fleckenstein, Lutz Foucar, Heinz Graafsma, Lars Gumprecht, Janos Hajdu, Christina Y Hampton, Andreas Hartmann, Robert Hartmann, Günter Hauser, Helmut Hirsemann, Peter Holl, Mark S Hunter, Stephan Kassemeyer, Richard A Kirian, Lukas Lomb, Filipe RNC Maia, Nils Kimmel, Andrew V Martin, Marc Messerschmidt, Christian Reich, Daniel Rolles, Benedikt Rudek, Artem Rudenko, Ilme Schlichting, Joachim Schulz, M Marvin Seibert, Robert L Shoeman, Raymond G Sierra, Heike Soltau, Stephan Stern, Lothar Strüder, Nicusor Timneanu, Joachim Ullrich, Xiaoyu Wang, Georg Weidenspointner, Uwe Weierstall, Garth J Williams, Cornelia B Wunderer, Petra Fromme, John CH Spence, Thilo Stehle, Henry N Chapman, Christian Betzel, and Michael Duszenko
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Summary:Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo-grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Item Description:Gesehen am 18.09.2018
Physical Description:Online Resource
ISSN:1548-7105
DOI:10.1038/nmeth.1859

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