Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range
Single molecule observation of fluorescence resonance energy transfer can be used to provide insights into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions wit...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
13 Jun 2012
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| In: |
Molecular BioSystems
Year: 2012, Volume: 8, Issue: 10, Pages: 2531-2534 |
| ISSN: | 1742-2051 |
| DOI: | 10.1039/c2mb25135c |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1039/c2mb25135c Verlag, Volltext: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441834/ 
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| Author Notes: | Sigrid Milles, Christine Koehler, Yann Gambin, Ashok A Deniz, and Edward A Lemke |
| Summary: | Single molecule observation of fluorescence resonance energy transfer can be used to provide insights into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsically disordered proteins. |
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| Item Description: | Gesehen am 21.09.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1742-2051 |
| DOI: | 10.1039/c2mb25135c |