Orthogonal spin labeling using click chemistry for in vitro and in vivo applications
Site-directed spin labeling for EPR- and NMR spectroscopy has mainly been achieved exploiting the specific reactivity of cysteines. For proteins with native cysteines or for in vivo applications, an alternative coupling strategy is required. In these cases click chemistry offers major benefits by pr...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2017
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| In: |
Journal of magnetic resonance
Year: 2016, Volume: 275, Pages: 38-45 |
| ISSN: | 1096-0856 |
| DOI: | 10.1016/j.jmr.2016.12.001 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1016/j.jmr.2016.12.001 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1090780716302555 
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| Author Notes: | Svetlana Kucher, Sergei Korneev, Swati Tyagi, Ronja Apfelbaum, Dina Grohmann, Edward A. Lemke, Johann P. Klare, Heinz-Jürgen Steinhoff, Daniel Klose |
| Summary: | Site-directed spin labeling for EPR- and NMR spectroscopy has mainly been achieved exploiting the specific reactivity of cysteines. For proteins with native cysteines or for in vivo applications, an alternative coupling strategy is required. In these cases click chemistry offers major benefits by providing a fast and highly selective, biocompatible reaction between azide and alkyne groups. Here, we establish click chemistry as a tool to target unnatural amino acids in vitro and in vivo using azide- and alkyne-functionalized spin labels. The approach is compatible with a variety of labels including reduction-sensitive nitroxides. Comparing spin labeling efficiencies from the copper-free with the strongly reducing copper(I)-catalyzed azide-alkyne click reaction, we find that the faster kinetics for the catalyzed reaction outrun reduction of the labile nitroxide spin labels and allow quantitative labeling yields within short reaction times. Inter-spin distance measurements demonstrate that the novel side chain is suitable for paramagnetic NMR- or EPR-based conformational studies of macromolecular complexes. |
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| Item Description: | Published online: 2 December 2016 Gesehen am 26.09.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1096-0856 |
| DOI: | 10.1016/j.jmr.2016.12.001 |