Cell perturbation screens for target identification by RNAi
Over the last decade, cell-based screening has become a powerful method in target identification and plays an important role both in basic research and drug discovery. The availability of whole genome sequences and improvements in cell-based screening techniques opened new avenues for high-throughpu...
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| Main Authors: | , |
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| Format: | Chapter/Article |
| Language: | English |
| Published: |
18 Juni 2012
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| In: |
Bioinformatics and Drug Discovery
Year: 2012, Pages: 1-13 |
| DOI: | 10.1007/978-1-61779-965-5_1 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1007/978-1-61779-965-5_1
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| Author Notes: | Kubilay Demir, Michael Boutros |
| Summary: | Over the last decade, cell-based screening has become a powerful method in target identification and plays an important role both in basic research and drug discovery. The availability of whole genome sequences and improvements in cell-based screening techniques opened new avenues for high-throughput experiments. Large libraries of RNA interference reagents available for many organisms allow the dissection of broad spectrum of cellular processes. Here, we describe the current state of the large-scale phenotype screening with a focus on cell-based screens. We underline the importance and provide details of screen design, scalability, performance, data analysis, and hit prioritization. Similar to classical high-throughput in vitro screens with defined-target approaches in the past, cell-based screens depend on a successful establishment of robust phenotypic assays, the ability to quantitatively measure phenotypic changes and bioinformatics methods for data analysis, integration, and interpretation. |
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| Item Description: | Gesehen am 16.10.2018 |
| Physical Description: | Online Resource |
| ISBN: | 9781617799655 |
| DOI: | 10.1007/978-1-61779-965-5_1 |