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Cell perturbation screens for target identification by RNAi

Over the last decade, cell-based screening has become a powerful method in target identification and plays an important role both in basic research and drug discovery. The availability of whole genome sequences and improvements in cell-based screening techniques opened new avenues for high-throughpu...

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Hauptverfasser: Demir, Kubilay (VerfasserIn) , Boutros, Michael (VerfasserIn)
Dokumenttyp: Kapitel/Artikel
Sprache:Englisch
Veröffentlicht: 18 Juni 2012
In: Bioinformatics and Drug Discovery
Year: 2012, Pages: 1-13
DOI:10.1007/978-1-61779-965-5_1
Online-Zugang:Verlag, Volltext: https://doi.org/10.1007/978-1-61779-965-5_1
Volltext
Verfasserangaben:Kubilay Demir, Michael Boutros

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520 |a Over the last decade, cell-based screening has become a powerful method in target identification and plays an important role both in basic research and drug discovery. The availability of whole genome sequences and improvements in cell-based screening techniques opened new avenues for high-throughput experiments. Large libraries of RNA interference reagents available for many organisms allow the dissection of broad spectrum of cellular processes. Here, we describe the current state of the large-scale phenotype screening with a focus on cell-based screens. We underline the importance and provide details of screen design, scalability, performance, data analysis, and hit prioritization. Similar to classical high-throughput in vitro screens with defined-target approaches in the past, cell-based screens depend on a successful establishment of robust phenotypic assays, the ability to quantitatively measure phenotypic changes and bioinformatics methods for data analysis, integration, and interpretation. 
650 4 |a Assay development 
650 4 |a Drug and RNAi screen design 
650 4 |a Functional genomics 
650 4 |a Hit prioritization 
650 4 |a Homogeneous and high-content screen analysis 
650 4 |a RNAi libraries 
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