GFP tagging sheds light on protein translocation: implications for key methods in cell biology
Green fluorescent protein (GFP) is a powerful tool for studying gene expression, protein localization, protein-protein interactions, calcium concentrations, and redox potentials owing to its intrinsic fluorescence. However, GFP not only contains a chromophore but is also tightly folded in a temperatur...
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| Format: | Article (Journal) |
| Language: | English |
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16 February 2012
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| In: |
Cellular and molecular life sciences
Year: 2012, Volume: 69, Issue: 7, Pages: 1025-1033 |
| ISSN: | 1420-9071 |
| DOI: | 10.1007/s00018-012-0932-6 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s00018-012-0932-6 Verlag, Volltext: http://link.springer.com/10.1007/s00018-012-0932-6 
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| Author Notes: | Marcel Deponte |
| Summary: | Green fluorescent protein (GFP) is a powerful tool for studying gene expression, protein localization, protein-protein interactions, calcium concentrations, and redox potentials owing to its intrinsic fluorescence. However, GFP not only contains a chromophore but is also tightly folded in a temperature-dependent manner. The latter property of GFP has recently been exploited (1) to characterize the translocase of the outer mitochondrial membrane and (2) to discriminate between protein transport across and into biomembranes in vivo. I therefore suggest that GFP could be a valuable tool for the general analysis of protein transport machineries and pathways in a variety of organisms. Moreover, results from such studies could be important for the interpretation and optimization of classical experiments using GFP tagging. |
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| Item Description: | Gesehen am 17.10.2018 Published 16 February 2012 |
| Physical Description: | Online Resource |
| ISSN: | 1420-9071 |
| DOI: | 10.1007/s00018-012-0932-6 |