Workflows for microscopy image analysis and cellular phenotyping

In large scale biological experiments, like high-throughput or high-content cellular screening, the amount and the complexity of images to be analyzed are steadily increasing. To handle and process these images, well defined image processing and analysis steps need to be performed by applying dedica...

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Bibliographic Details
Main Authors: Wollmann, Thomas (Author) , Erfle, Holger (Author) , Eils, Roland (Author) , Rohr, Karl (Author) , Gunkel, Manuel (Author)
Format: Article (Journal)
Language:English
Published: 27 July 2017
In: Journal of biotechnology
Year: 2017, Volume: 261, Pages: 70-75
ISSN:1873-4863
DOI:10.1016/j.jbiotec.2017.07.019
Online Access:Verlag, Volltext: http://dx.doi.org/10.1016/j.jbiotec.2017.07.019
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0168165617315444
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Author Notes:Thomas Wollmann, Holger Erfle, Roland Eils, Karl Rohr, Manuel Gunkel
Description
Summary:In large scale biological experiments, like high-throughput or high-content cellular screening, the amount and the complexity of images to be analyzed are steadily increasing. To handle and process these images, well defined image processing and analysis steps need to be performed by applying dedicated workflows. Multiple software tools have emerged with the aim to facilitate creation of such workflows by integrating existing methods, tools, and routines, and by adapting them to different applications and questions, as well as making them reusable and interchangeable. In this review, we describe workflow systems for the integration of microscopy image analysis techniques with focus on KNIME and Galaxy.
Item Description:Gesehen am 23.10.2018
Physical Description:Online Resource
ISSN:1873-4863
DOI:10.1016/j.jbiotec.2017.07.019