Hydroxylated fluorescent dyes for live-cell labeling: synthesis, spectra and super-resolution STED
Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
September 7, 2017
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| In: |
Chemistry - a European journal
Year: 2017, Volume: 23, Issue: 50, Pages: 12114-12119 |
| ISSN: | 1521-3765 |
| DOI: | 10.1002/chem.201701216 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1002/chem.201701216 Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.201701216 
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| Author Notes: | Alexey N. Butkevich, Vladimir N. Belov, Kirill Kolmakov, Viktor V. Sokolov, Heydar Shojaei, Sven C. Sidenstein, Dirk Kamin, Jessica Matthias, Rifka Vlijm, Johann Engelhardt, Stefan W. Hell |
| Summary: | Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow for prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogues of rhodamines, carbo-, silico-, and germanorhodamines using simple additive schemes. |
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| Item Description: | First published: 29 March 2017 Gesehen am 24.10.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1521-3765 |
| DOI: | 10.1002/chem.201701216 |