In vitro N-Acetyl-l-Cysteine promotes proliferation and suppresses interleukin-8 expression in adipose-derived stem cells
Adipose-derived stem cells (ADSCs) hold great promise for repair and regeneration of burn wounds by producing growth factors, but proinflammatory cytokines such as interleukin-8 (IL-8) released by ADSCs would potentially deepen the wound and inhibit healing. The reported research aimed to identify t...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
31 August 2012
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| In: |
Aesthetic plastic surgery
Year: 2012, Volume: 36, Issue: 5, Pages: 1260-1265 |
| ISSN: | 1432-5241 |
| DOI: | 10.1007/s00266-012-9960-8 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1007/s00266-012-9960-8 Verlag, Volltext: https://doi.org/10.1007/s00266-012-9960-8 
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| Author Notes: | Lingyun Xiong, Jiaming Sun, Christoph Hirche, Jie Yang, Yanqing Yang, Yun Xia, Marcus Lehnhardt, Rongrong Wang, Xi Fu |
| Summary: | Adipose-derived stem cells (ADSCs) hold great promise for repair and regeneration of burn wounds by producing growth factors, but proinflammatory cytokines such as interleukin-8 (IL-8) released by ADSCs would potentially deepen the wound and inhibit healing. The reported research aimed to identify the effects of N-acetyl-l-cysteine (NAC) on the proliferation, death, and IL-8 production of ADSCs. In the presence or absence of NAC, ADSC proliferation was examined using a CCK-8 Kit, and cell death was evaluated by flow cytometry analysis. Subsequently, IL-8 mRNA expression was detected by reverse transcriptase-polymerase chain reaction and protein production by enzyme-linked immunoassay. Findings showed that cell proliferation in the NAC-treated group was a significant 1.53-fold greater than in the control group, that the apoptosis rate of ADSCs decreased by 55.4 % compared with the control group, and that the necrosis rate decreased by 48.8 %. Additionally, the IL-8 mRNA expression decreased to 46.2 ± 8.7 % that of the control group, and the IL-8 protein production decreased to 9.98 ± 0.57 %. The authors believe that NAC might be helpful in burn wound repair and regeneration by stimulating the proliferation of ADSCs, inhibiting cell death, and suppressing IL-8 production.Level of Evidence IIThis journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. |
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| Item Description: | Gesehen am 25.10.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1432-5241 |
| DOI: | 10.1007/s00266-012-9960-8 |