Two-color 810 nm STED nanoscopy of living cells with endogenous SNAP-tagged fusion proteins
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncov...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
21 September 2017
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| In: |
ACS chemical biology
Year: 2018, Volume: 13, Issue: 2, Pages: 475-480 |
| ISSN: | 1554-8937 |
| DOI: | 10.1021/acschembio.7b00616 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1021/acschembio.7b00616 Verlag, Volltext: https://doi.org/10.1021/acschembio.7b00616 
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| Author Notes: | Alexey N. Butkevich, Haisen Ta, Michael Ratz, Stefan Stoldt, Stefan Jakobs, Vladimir N. Belov, and Stefan W. Hell |
| Summary: | A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells. |
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| Item Description: | Published online 2 October 2017 Gesehen am 25.10.2018 |
| Physical Description: | Online Resource |
| ISSN: | 1554-8937 |
| DOI: | 10.1021/acschembio.7b00616 |