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Distinct subsets of syt-IV/BDNF vesicles are sorted to axons versus dendrites and recruited to synapses by activity

BDNF plays a critical role in the regulation of synaptic strength and is essential for long-term potentiation, a phenomenon that underlies learning and memory. However, whether BDNF acts in a diffuse manner or is targeted to specific neuronal subcompartments or synaptic sites to affect circuit funct...

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Main Authors: Dean, Camin (Author) , Staudt, Thorsten M. (Author) , Engelhardt, Johann (Author) , Hell, Stefan (Author)
Format: Article (Journal)
Language:English
Published: 18 April 2012
In: The journal of neuroscience
Year: 2012, Volume: 32, Issue: 16, Pages: 5398-5413
ISSN:1529-2401
DOI:10.1523/JNEUROSCI.4515-11.2012
Online Access:Verlag, Volltext: http://dx.doi.org/10.1523/JNEUROSCI.4515-11.2012
Verlag, Volltext: http://www.jneurosci.org/content/32/16/5398
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Author Notes:Camin Dean, Huisheng Liu, Thorsten Staudt, Markus A. Stahlberg, Siv Vingill, Johanna Bückers, Dirk Kamin, Johann Engelhardt, Meyer B. Jackson, Stefan W. Hell, and Edwin R. Chapman
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Summary:BDNF plays a critical role in the regulation of synaptic strength and is essential for long-term potentiation, a phenomenon that underlies learning and memory. However, whether BDNF acts in a diffuse manner or is targeted to specific neuronal subcompartments or synaptic sites to affect circuit function remains unknown. Here, using photoactivation of BDNF or syt-IV (a regulator of exocytosis present on BDNF-containing vesicles) in transfected rat hippocampal neurons, we discovered that distinct subsets of BDNF vesicles are targeted to axons versus dendrites and are not shared between these compartments. Moreover, syt-IV- and BDNF-harboring vesicles are recruited to both presynaptic and postsynaptic sites in response to increased neuronal activity. Finally, using syt-IV knockout mouse neurons, we found that syt-IV is necessary for both presynaptic and postsynaptic scaling of synaptic strength in response to changes in network activity. These findings demonstrate that BDNF-containing vesicles can be targeted to specific sites in neurons and suggest that syt-IV-regulated BDNF secretion is subject to spatial control to regulate synaptic function in a site-specific manner.
Item Description:Gesehen am 29.10.2018
Physical Description:Online Resource
ISSN:1529-2401
DOI:10.1523/JNEUROSCI.4515-11.2012

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