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Genome-wide analysis of somatic copy number alterations and chromosomal breakages in osteosarcoma

Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. It is characterized by highly complex karyotypes with structural and numerical chromosomal alterations. The observed OS-specific characteristics in localization and frequencies of chromosomal breakages str...

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Main Authors: Smida, Jan (Author) , Xu, Hongen (Author) , Zhang, Yanping (Author) , Baumhoer, Daniel (Author) , Ribi, Sebastian (Author) , Kovac, Michal (Author) , Teichert- von Lüttichau, Irene (Author) , Bielack, Stefan (Author) , O'Leary, Valerie B. (Author) , Leib-Mösch, Christine (Author) , Frishman, Dmitij (Author) , Nathrath, Michaela (Author)
Format: Article (Journal)
Language:English
Published: 11 May 2017
In: International journal of cancer
Year: 2017, Volume: 141, Issue: 4, Pages: 816-828
ISSN:1097-0215
DOI:10.1002/ijc.30778
Online Access:Verlag, Volltext: http://dx.doi.org/10.1002/ijc.30778
Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.30778
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Author Notes:Jan Smida, Hongen Xu, Yanping Zhang, Daniel Baumhoer, Sebastian Ribi, Michal Kovac, Irene von Luettichau, Stefan Bielack, Valerie B. O'Leary, Christine Leib‐Mösch, Dmitrij Frishman and Michaela Nathrath
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Summary:Osteosarcoma (OS) is the most common primary malignant bone tumor in children and adolescents. It is characterized by highly complex karyotypes with structural and numerical chromosomal alterations. The observed OS-specific characteristics in localization and frequencies of chromosomal breakages strongly implicate a specific set of responsible driver genes or a specific mechanism of fragility induction. In this study, a comprehensive assessment of somatic copy number alterations (SCNAs) was performed in 160 OS samples using whole-genome CytoScan High Density arrays (Affymetrix, Santa Clara, CA). Genes or regions frequently targeted by SCNAs were identified. Breakage analysis revealed OS specific unstable regions in which well-known OS tumor suppressor genes, including TP53, RB1, WWOX, DLG2 and LSAMP are located. Certain genomic features, such as transposable elements and non-B DNA-forming motifs were found to be significantly enriched in the vicinity of chromosomal breakage sites. A complex breakage pattern - chromothripsis - has been suggested as a widespread phenomenon in OS. It was further demonstrated that hyperploidy and in particular chromothripsis were strongly correlated with OS patient clinical outcome. The revealed OS-specific fragility pattern provides novel clues for understanding the biology of OS.
Item Description:Gesehen am 05.11.2018
Physical Description:Online Resource
ISSN:1097-0215
DOI:10.1002/ijc.30778

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