rsEGFP2 enables fast RESOLFT nanoscopy of living cells
The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchab...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
31 December 2012
|
| In: |
eLife
Year: 2012, Volume: 1 |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.00248 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.7554/eLife.00248 Verlag, kostenfrei, Volltext: https://doi.org/10.7554/eLife.00248 
|
| Author Notes: | Tim Grotjohann, Ilaria Testa, Matthias Reuss, Tanja Brakemann, Christian Eggeling, Stefan W Hell, Stefan Jakobs |
| Summary: | The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings. |
|---|---|
| Item Description: | Gesehen am 05.11.2018 |
| Physical Description: | Online Resource |
| ISSN: | 2050-084X |
| DOI: | 10.7554/eLife.00248 |