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Wnt-11 and Fz7 reduce cell adhesion in convergent extension by sequestration of PAPC and C-cadherin

Wnt-11/planar cell polarity signaling polarizes mesodermal cells undergoing convergent extension during Xenopus laevis gastrulation. These shape changes associated with lateral intercalation behavior require a dynamic modulation of cell adhesion. In this paper, we report that Wnt-11/frizzled-7 (Fz7)...

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Bibliographic Details
Main Authors: Kraft, Bianca (Author) , Berger, Corinna D. (Author) , Steinbeisser, Herbert (Author)
Format: Article (Journal)
Language:English
Published: August 20, 2012
In: The journal of cell biology
Year: 2012, Volume: 198, Issue: 4, Pages: 695-709
ISSN:1540-8140
DOI:10.1083/jcb.201110076
Online Access:Verlag, Volltext: http://dx.doi.org/10.1083/jcb.201110076
Verlag, Volltext: http://jcb.rupress.org/content/198/4/695
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Author Notes:Bianca Kraft, Corinna D. Berger, Veronika Wallkamm, Herbert Steinbeisser, and Doris Wedlich
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Summary:Wnt-11/planar cell polarity signaling polarizes mesodermal cells undergoing convergent extension during Xenopus laevis gastrulation. These shape changes associated with lateral intercalation behavior require a dynamic modulation of cell adhesion. In this paper, we report that Wnt-11/frizzled-7 (Fz7) controls cell adhesion by forming separate adhesion-modulating complexes (AMCs) with the paraxial protocadherin (PAPC; denoted as AMCP) and C-cadherin (denoted as AMCC) via distinct Fz7 interaction domains. When PAPC was part of a Wnt-11-Fz7 complex, its Dynamin1- and clathrin-dependent internalization was blocked. This membrane stabilization of AMCP (Fz7/PAPC) by Wnt-11 prevented C-cadherin clustering, resulting in reduced cell adhesion and modified cell sorting activity. Importantly, Wnt-11 did not influence C-cadherin internalization; instead, it promoted the formation of AMCC (Fz7/Cadherin), which competed with cis-dimerization of C-cadherin. Because PAPC and C-cadherin did not directly interact and did not form a joint complex with Fz7, we suggest that Wnt-11 triggers the formation of two distinct complexes, AMCC and AMCP, that act in parallel to reduce cell adhesion by hampering lateral clustering of C-cadherin.
Item Description:Gesehen am 08.11.2018
Physical Description:Online Resource
ISSN:1540-8140
DOI:10.1083/jcb.201110076

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