Cover Image

Reaction of a programmable glycan presentation of glycodendrimersomes and cells with engineered human lectins to show the sugar functionality of the cell surface

Chemical and biological tools are harnessed to investigate the impact of spatial factors for functional pairing of human lectins with counterreceptors. The homodimeric adhesion/growth-regulatory galectin-1 and a set of covalently linked homo-oligomers from di- to tetramers serve as proof-of-principl...

Full description

Saved in:
Bibliographic Details
Main Authors: Kopitz, Jürgen (Author) , Michalak, Malwina (Author)
Format: Article (Journal)
Language:English
Published: November 13, 2017
In: Angewandte Chemie. International edition
Year: 2017, Volume: 56, Issue: 46, Pages: 14677-14681
ISSN:1521-3773
DOI:10.1002/anie.201708237
Online Access:Verlag, Volltext: http://dx.doi.org/10.1002/anie.201708237
Verlag, Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201708237
Get full text
Author Notes:Jürgen Kopitz, Qi Xiao, Anna-Kristin Ludwig, Antonio Romero, Malwina Michalak, Samuel E. Sherman, Xuhao Zhou, Cody Dazen, Sabine Vértesy, Herbert Kaltner, Michael L. Klein, Hans-Joachim Gabius, and Virgil Percec
Description
Summary:Chemical and biological tools are harnessed to investigate the impact of spatial factors for functional pairing of human lectins with counterreceptors. The homodimeric adhesion/growth-regulatory galectin-1 and a set of covalently linked homo-oligomers from di- to tetramers serve as proof-of-principle test cases. Glycodendrimersomes provide a versatile and sensitive diagnostic platform to reveal thresholds for ligand density and protein concentration in aggregation assays (trans-activity), irrespective of linker length between lectin domains. Monitoring the affinity of cell binding and ensuing tumor growth inhibition reveal the linker length to be a bidirectional switch for cis-activity. The discovery that two aspects of lectin functionality (trans- versus cis-activity) respond non-uniformly to a structural change underscores the power of combining synthetic and biological tools to advance understanding of the sugar functionality of the cell surface.
Item Description:Gesehen am 14.11.2018
Physical Description:Online Resource
ISSN:1521-3773
DOI:10.1002/anie.201708237

Similar Items