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Three critical hydrogen bonds determine the catalytic activity of the Diels-Alderase ribozyme

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Abstract. Compared to protein enzymes, our knowledge about how RNA accelerates chemical reactions is rather limited. The crystal structures of a ribozyme that catalyzes Diels–Alder reactions suggest a rich tertiary architecture responsible for catalysis. In this study, we systematically probe the r...

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Bibliographic Details
Main Authors: Kraut, Stefanie (Author) , Bebenroth, Dirk (Author) , Nierth, Alexander (Author) , Jäschke, Andres (Author)
Format: Article (Journal)
Language:English
Published: 1 February 2012
In: Nucleic acids research
Year: 2012, Volume: 40, Issue: 3, Pages: 1318-1330
ISSN:1362-4962
DOI:10.1093/nar/gkr812
Online Access:Verlag, Volltext: http://dx.doi.org/10.1093/nar/gkr812
Verlag, Volltext: https://academic.oup.com/nar/article/40/3/1318/1137573
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Author Notes:Stefanie Kraut, Dirk Bebenroth, Alexander Nierth, Andrei Y. Kobitski, G. Ulrich Nienhaus and Andres Jäschke
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Summary:Abstract. Compared to protein enzymes, our knowledge about how RNA accelerates chemical reactions is rather limited. The crystal structures of a ribozyme that catalyzes Diels–Alder reactions suggest a rich tertiary architecture responsible for catalysis. In this study, we systematically probe the relevance of crystallographically observed ground-state interactions for catalytic function using atomic mutagenesis in combination with various analytical techniques.
Item Description:Published online 5 October 2011
Gesehen am 19.11.2018
Physical Description:Online Resource
ISSN:1362-4962
DOI:10.1093/nar/gkr812

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