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Hematopoietic stem cells can be separated from leukemic cells in a subgroup of adult acute lymphoblastic leukemia patients

In B-cell acute lymphoblastic leukemia (B-ALL) separation of normal hematopoietic stem cells (HSC) has so far been limited to a subgroup of patients. As aldehyde dehydrogenase (ALDH)-activity is enriched in various stem cells we investigated its value for HSC isolation in adult B-ALL. Based on ALDH-...

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Main Authors: Wang, Wenwen (Author) , Buß, Eike Christian (Author) , Jauch, Anna (Author) , Eckstein, Volker (Author) , Wuchter, Patrick (Author) , Ho, Anthony Dick (Author) , Lutz, Christoph (Author)
Format: Article (Journal)
Language:English
Published: 2017
In: Leukemia and lymphoma
Year: 2017, Volume: 58, Issue: 6, Pages: 1446-1454
ISSN:1029-2403
DOI:10.1080/10428194.2016.1236378
Online Access:Verlag, Volltext: http://dx.doi.org/10.1080/10428194.2016.1236378
Verlag, Volltext: https://doi.org/10.1080/10428194.2016.1236378
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Author Notes:Wenwen Wang, Elena Foerner, Eike Buss, Anna Jauch, Volker Eckstein, Patrick Wuchter, Anthony D. Ho, Christoph Lutz
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Summary:In B-cell acute lymphoblastic leukemia (B-ALL) separation of normal hematopoietic stem cells (HSC) has so far been limited to a subgroup of patients. As aldehyde dehydrogenase (ALDH)-activity is enriched in various stem cells we investigated its value for HSC isolation in adult B-ALL. Based on ALDH-activity patients could be stratified in ALDH-numerous (≥1.9% ALDH+ cells) and ALDH-rare (<1.9% ALDH+ cells) cases. In ALDH-rare B-ALL clonal-marker negative HSC could be separated by the CD34+CD38−ALDH+ phenotype, whereas this separation was not possible in ALDH-numerous B-ALL. Functional analysis confirmed the HSC-potential of isolated cells, which were uniformly CD19-negative. However, addition of ALDH-activity further improved HSC-purity. In summary, we provide a method to separate functionally normal HSC from leukemic cells in a subgroup of B-ALL patients that can be identified prospectively. This protocol thereby facilitates comparative analyses of matched HSC and leukemic cells in order to improve our understanding of leukemia evolution.
Item Description:Published online 13 Oct 2016
Gesehen am 22.11.2018
Physical Description:Online Resource
ISSN:1029-2403
DOI:10.1080/10428194.2016.1236378

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