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Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9

Anti-CRISPR proteins are powerful tools for CRISPR–Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogenetic anti-CRISPR variants comprising hybrids of...

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Main Authors: Bubeck, Felix (Author) , Hoffmann, Mareike (Author) , Bietz, Andreas (Author) , Waldhauer, Max (Author) , Börner, Kathleen (Author) , Fakhiri, Julia (Author) , Schmelas, Carolin (Author) , Dietz, Laura (Author) , Grimm, Dirk (Author) , Eils, Roland (Author) , Niopek, Dominik (Author)
Format: Article (Journal)
Language:English
Published: 30 October 2018
In: Nature methods
Year: 2018, Volume: 15, Issue: 11, Pages: 924-927
ISSN:1548-7105
DOI:10.1038/s41592-018-0178-9
Online Access:Resolving-System, Volltext: http://dx.doi.org/10.1038/s41592-018-0178-9
Verlag, Volltext: https://www.nature.com/articles/s41592-018-0178-9
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Author Notes:Felix Bubeck, Mareike D. Hoffmann, Zander Harteveld, Sabine Aschenbrenner, Andreas Bietz, Max C. Waldhauer, Kathleen Börner, Julia Fakhiri, Carolin Schmelas, Laura Dietz, Dirk Grimm, Bruno E. Correia, Roland Eils and Dominik Niopek
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Summary:Anti-CRISPR proteins are powerful tools for CRISPR–Cas9 regulation; the ability to precisely modulate their activity could facilitate spatiotemporally confined genome perturbations and uncover fundamental aspects of CRISPR biology. We engineered optogenetic anti-CRISPR variants comprising hybrids of AcrIIA4, a potent Streptococcus pyogenes Cas9 inhibitor, and the LOV2 photosensor from Avena sativa. Coexpression of these proteins with CRISPR–Cas9 effectors enabled light-mediated genome and epigenome editing, and revealed rapid Cas9 genome targeting in human cells.
Item Description:Das PDF enthält 4 Seiten Text und 6 Seiten Anhang
Gesehen am 03.12.2018
Physical Description:Online Resource
ISSN:1548-7105
DOI:10.1038/s41592-018-0178-9

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