CpG-oligodeoxynucleotides in chronic rhinosinusitis cell culture
In chronic rhinosinusitis (CRS) an important feature is the infiltration of eosinophils, triggered by T-helper type 2 cells (TH2). Binding of the CpG oligodeoxynucleotide (CpG-ODN) ligand to toll-like receptor 9 (TLR9) induces a shift from a TH2- to a TH1-type response. We evaluated the hypothesis t...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
January-February 2016
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| In: |
In vivo
Year: 2016, Jahrgang: 30, Heft: 1, Pages: 47-52 |
| ISSN: | 1791-7549 |
| Online-Zugang: | Verlag, Volltext: http://iv.iiarjournals.org/content/30/1/47
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| Verfasserangaben: | Richard Birk, Christoph Aderhold, Karl Hörmann, Angela Wenzel, Benedikt Kramer, Till Eschenhagen and J. Ulrich Sommer |
| Zusammenfassung: | In chronic rhinosinusitis (CRS) an important feature is the infiltration of eosinophils, triggered by T-helper type 2 cells (TH2). Binding of the CpG oligodeoxynucleotide (CpG-ODN) ligand to toll-like receptor 9 (TLR9) induces a shift from a TH2- to a TH1-type response. We evaluated the hypothesis that CpG-ODN could reduce the predominantly TH2-driven response in our cultures. Materials and Methods: Twenty samples from CRS patients with (CRSwNP) and without nasal polyposis (CRSsNP) were cultivated. The expression of interleukin 5 (IL-5), eotaxin 3 and matrix metalloprotease 9 (MMP-9) were evaluated with and without CpG-ODN. Results: Addition of CpG did not influence the expression of IL-5 and eotaxin-3 DNA. Elevated MMP-9 expression in cultures from CRSwNP and CRSsNP patients could be established. Conclusion: CpG does not reduce the attraction of eosinophils since no reduced IL-5 expression was measured in our cultures. Yet, MMP-9 - an important factor in tissue remodelling - was elevated in cultures from CRS patients. |
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| Beschreibung: | Gesehen am 23.01.2019 |
| Beschreibung: | Online Resource |
| ISSN: | 1791-7549 |