Retinal digest preparation: a method to study diabetic retinopathy

Retinal digestion is a commonly used method for studying experimental diabetic retinopathy in animal models. The method allows to assess qualitatively and quantitatively the morphology of the retinal vasculature, including characteristics of endothelial cells and pericytes. The digestion method uses...

Full description

Saved in:
Bibliographic Details
Main Authors: Dietrich, Nadine (Author) , Hammes, Hans-Peter (Author)
Format: Chapter/Article
Language:English
Published: 18 July 2012
In: Animal Models in Diabetes Research
Year: 2012, Pages: 291-302
DOI:10.1007/978-1-62703-068-7_19
Online Access:Verlag, Volltext: https://doi.org/10.1007/978-1-62703-068-7_19
Get full text
Author Notes:Nadine Dietrich, Hans-Peter Hammes
Description
Summary:Retinal digestion is a commonly used method for studying experimental diabetic retinopathy in animal models. The method allows to assess qualitatively and quantitatively the morphology of the retinal vasculature, including characteristics of endothelial cells and pericytes. The digestion method uses the enzyme trypsin and enables the precise evaluation of venolar and arteriolar diameters, endothelial cell and pericyte numbers, and the formation of acellular capillaries.
Item Description:Gesehen am 13.02.2019
Physical Description:Online Resource
ISBN:9781627030687
DOI:10.1007/978-1-62703-068-7_19