Comparison of non-viral transfection methods in melanoma cell primary cultures
Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58±9%) as compared to lipofection (23±9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 p...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2002
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| In: |
Cytokine
Year: 2000, Volume: 12, Issue: 6, Pages: 828-833 |
| ISSN: | 1096-0023 |
| DOI: | 10.1006/cyto.1999.0621 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1006/cyto.1999.0621 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S104346669990621X 
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| Author Notes: | Bettina Micka, Beate Trojaneck, Sigrun Niemitz, Petja Lefterova, Susanne Kruopis, Dieter Huhn, Burghardt Wittig, Dirk Schadendorf, Ingo G.H. Schmidt-Wolf |
| Summary: | Melanoma primary cultures were transiently transfected via electroporation and lipofection for comparison. Transfection efficiency was superior with electroporation (58±9%) as compared to lipofection (23±9%) as determined by enhanced green fluorescent plasmid (EGFP) transfection. Secretion of IL-2 persisted for up to 3 weeks after electroporation. The increase in sensitivity against immunologic effector cells by transfection with IL-2 was not significant. Our results show the feasibility of a gene tranfer into primary human melanoma cells, different from retroviral transduction. |
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| Item Description: | Available online 25 May 2002 Gesehen am 26.02.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1096-0023 |
| DOI: | 10.1006/cyto.1999.0621 |