Protein labeling with the labeling precursor [18F]SiFA-SH for positron emission tomography
Proteins previously derivatized with the cross-coupling reagent sulfo-SMCC (4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxy-succinimide ester sodium salt) can be easily labeled in high radiochemical yields with the silicon-fluoride acceptor (SiFA) reagent [18F]SiFA-SH, obtained...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
04 October 2012
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| In: |
Nature protocols
Year: 2012, Volume: 7, Issue: 11, Pages: 1964-1969 |
| ISSN: | 1750-2799 |
| DOI: | 10.1038/nprot.2012.111 |
| Online Access: | Verlag, Volltext: http://dx.doi.org/10.1038/nprot.2012.111 Verlag, Volltext: https://www.nature.com/articles/nprot.2012.111 
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| Author Notes: | Björn Wängler, Alexey P. Kostikov, Sabrina Niedermoser, Joshua Chin, Katy Orchowski, Esther Schirrmacher, Liuba Iovkova-Berends, Klaus Jurkschat, Carmen Wängler, Ralf Schirrmacher |
| Summary: | Proteins previously derivatized with the cross-coupling reagent sulfo-SMCC (4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxy-succinimide ester sodium salt) can be easily labeled in high radiochemical yields with the silicon-fluoride acceptor (SiFA) reagent [18F]SiFA-SH, obtained via isotopic exchange, by thiol-maleimide coupling chemistry (n = 10). The specific activity of SiFA-SH obtained in a one-step labeling reaction was >18.5 GBq μmol−1 (>500 Ci mmol−1). The number of SiFA building blocks per protein molecule is defined by the previously introduced number of maleimide groups, which can be determined by a simple and convenient Ellman's assay. Not more than two maleimide groups are introduced using sulfo-SMCC, thereby keeping the modification of the protein low and preserving its biological activity. |
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| Item Description: | Im Titel ist die Zahl 18 hochgestellt Gesehen am 27.02.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1750-2799 |
| DOI: | 10.1038/nprot.2012.111 |