Innate DNA and RNA recognition: methods and protocols
Detection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-l...
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| Other Authors: | , |
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| Format: | Book/Monograph |
| Language: | English |
| Published: |
New York, NY
Humana Press
2014
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| Series: | Methods in molecular biology
1169 |
| In: |
Methods in molecular biology (1169)
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| Volumes / Articles: | Show Volumes / Articles. |
| DOI: | 10.1007/978-1-4939-0882-0 |
| Online Access: | Resolving-System, lizenzpflichtig: https://doi.org/10.1007/978-1-4939-0882-0 Cover: https://swbplus.bsz-bw.de/bsz409580139cov.jpg 
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| Author Notes: | edited by Hans-Joachim Anders, Adriana Migliorini |
| Summary: | Detection of RNA Modifications by HPLC Analysis and Competitive ELISA -- Enzymatic Synthesis and Purification of a Defined RIG-I Ligand -- Crystallization of Mouse RIG-I ATPase Domain: In situ Proteolysis -- Isolation of RIG-I-associated RNAs from Virus Infected Cells -- Structure Modeling of Toll-like Receptors -- Nucleic Acid Recognition in Dendritic Cell -- Viral Nucleic Acid Recognition in Human Non-immune cells: In vitro Systems -- Analysis of Nucleic Acid-induced Non-immune Cell Death in Vitro -- In vitro Analysis of Nucleic Acid Recognition in B Lymphocytes -- Mapping of Optimal CD8 T Cell Epitopes -- A Modular Approach to Suppression Ass says: TLR-Ligands, Conditioned Medium and Viral Infection -- MicroRNA Methodology: Advances in miRNA-technologies -- Expression Profiling by Real-time Quantitative Polymerase Chain Reaction (RT-qPCR) -- Evaluating the Role of Nucleic Acid Antigens in Murine Models of Systemic Lupus Erythematosus -- Induction and Analysis of Nephrotoxic Serum Nephritis in Mice -- Isolation of Intratumoral Leukocytes of TLR-stimulated Tumor-bearing Mice -- Bifunctional siRNAs for Tumor Therapy. Innate DNA and RNA Recognition: Method and Protocols presents validated experimental strategies to dissect nucleic acid sensing in-vitro and in-vivo sources. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate DNA and RNA Recognition: Method and Protocols provides a resource for immunologists, molecular biologists, virologists, microbiologists, and researchers studying how the innate immune system handles nucleic acids from endogenous or foreign sources. |
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| Physical Description: | Online Resource |
| ISBN: | 9781493908820 |
| DOI: | 10.1007/978-1-4939-0882-0 |