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Rapid proteomic analysis for solid tumors reveals LSD1 as a drug target in an end-stage cancer patient

Recent advances in mass spectrometry (MS)-based technologies are now set to transform translational cancer proteomics from an idea to a practice. Here, we present a robust proteomic workflow for the analysis of clinically relevant human cancer tissues that allows quantitation of thousands of tumor p...

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Bibliographic Details
Main Authors: Doll, Sophia (Author) , Kriegmair, Maximilian (Author) , Porubský, Štefan (Author) , Nuhn, Philipp (Author)
Format: Article (Journal)
Language:English
Published: 14 June 2018
In: Molecular oncology
Year: 2018, Volume: 12, Issue: 8, Pages: 1296-1307
ISSN:1878-0261
DOI:10.1002/1878-0261.12326
Online Access:Verlag, Volltext: https://doi.org/10.1002/1878-0261.12326
Verlag, Volltext: https://febs.onlinelibrary.wiley.com/doi/abs/10.1002/1878-0261.12326
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Author Notes:Sophia Doll, Maximilian C. Kriegmair, Alberto Santos, Michael Wierer, Fabian Coscia, Helen Michele Neil, Stefan Porubsky, Philipp E. Geyer, Andreas Mund, Philipp Nuhn and Matthias Mann
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Summary:Recent advances in mass spectrometry (MS)-based technologies are now set to transform translational cancer proteomics from an idea to a practice. Here, we present a robust proteomic workflow for the analysis of clinically relevant human cancer tissues that allows quantitation of thousands of tumor proteins in several hours of measuring time and a total turnaround of a few days. We applied it to a chemorefractory metastatic case of the extremely rare urachal carcinoma. Quantitative comparison of lung metastases and surrounding tissue revealed several significantly upregulated proteins, among them lysine-specific histone demethylase 1 (LSD1/KDM1A). LSD1 is an epigenetic regulator and the target of active development efforts in oncology. Thus, clinical cancer proteomics can rapidly and efficiently identify actionable therapeutic options. While currently described for a single case study, we envision that it can be applied broadly to other patients in a similar condition.
Item Description:Gesehen am 10.04.2019
Physical Description:Online Resource
ISSN:1878-0261
DOI:10.1002/1878-0261.12326

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