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Decoding the regulatory logic of the Drosophila male stem cell system

Summary - The niche critically controls stem cell behavior, but its regulatory input at the whole-genome level is poorly understood. We elucidated transcriptional programs of the somatic and germline lineages in the Drosophila testis and genome-wide binding profiles of Zfh-1 and Abd-A expressed in s...

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Main Authors: Tamirisa, Srividya (Author) , Papagiannouli, Fani (Author) , Rempel, Eugen (Author) , Ermakova, Olga (Author) , Trost, Nils (Author) , Zhou, Jun (Author) , Ruhland, Naima (Author) , Boutros, Michael (Author) , Lohmann, Jan U. (Author) , Lohmann, Ingrid (Author)
Format: Article (Journal)
Language:English
Published: September 11, 2018
In: Cell reports
Year: 2018, Volume: 24, Issue: 11, Pages: 3072-3086
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.08.013
Online Access:Verlag, Volltext: https://doi.org/10.1016/j.celrep.2018.08.013
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124718312671
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Author Notes:Srividya Tamirisa, Fani Papagiannouli, Eugen Rempel, Olga Ermakova, Nils Trost, Jun Zhou, Juliane Mundorf, Samantha Brunel, Naima Ruhland, Michael Boutros, Jan U. Lohmann, Ingrid Lohmann
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Summary:Summary - The niche critically controls stem cell behavior, but its regulatory input at the whole-genome level is poorly understood. We elucidated transcriptional programs of the somatic and germline lineages in the Drosophila testis and genome-wide binding profiles of Zfh-1 and Abd-A expressed in somatic support cells and crucial for fate acquisition of both cell lineages. We identified key roles of nucleoporins and V-ATPase proton pumps and demonstrate their importance in controlling germline development from the support side. To make our dataset publicly available, we generated an interactive analysis tool, which uncovered conserved core genes of adult stem cells across species boundaries. We tested the functional relevance of these genes in the Drosophila testis and intestine and found a high frequency of stem cell defects. In summary, our dataset and interactive platform represent versatile tools for identifying gene networks active in diverse stem cell types.
Item Description:Gesehen am 07.05.2019
Physical Description:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.08.013

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