Cover Image

Cathepsin G activity as a new marker for detecting airway inflammation by microscopy and flow cytometry

Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The...

Full description

Saved in:
Bibliographic Details
Main Authors: Guerra, Matteo (Author) , Frey, Dario (Author) , Hagner, Matthias (Author) , Dittrich, A. Susanne (Author) , Paulsen, Michelle (Author) , Mall, Marcus A. (Author) , Schultz, Carsten (Author)
Format: Article (Journal)
Language:English
Published: February 19, 2019
In: ACS central science
Year: 2019, Volume: 5, Issue: 3, Pages: 539-548
ISSN:2374-7951
DOI:10.1021/acscentsci.8b00933
Online Access:Verlag, Volltext: https://doi.org/10.1021/acscentsci.8b00933
Get full text
Author Notes:Matteo Guerra, Dario Frey, Matthias Hagner, Susanne Dittrich, Michelle Paulsen, Marcus A. Mall and Carsten Schultz
Description
Summary:Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The probes quantitatively revealed elevated CG activity in samples from 46 patients. For future basic science and personalized clinical applications, we developed a rapid, highly informative, and easily translatable small-molecule FRET flow cytometry assay for monitoring protease activity including cathepsin G. We demonstrated that mSAM distinguished healthy from patient cells by FRET-based flow cytometry with excellent correlation to confocal microscopy data.
Item Description:Gesehen am 27.05.2019
Physical Description:Online Resource
ISSN:2374-7951
DOI:10.1021/acscentsci.8b00933

Similar Items