Cathepsin G activity as a new marker for detecting airway inflammation by microscopy and flow cytometry
Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The...
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| Hauptverfasser: | , , , , , , |
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| Dokumenttyp: | Article (Journal) |
| Sprache: | Englisch |
| Veröffentlicht: |
February 19, 2019
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| In: |
ACS central science
Year: 2019, Jahrgang: 5, Heft: 3, Pages: 539-548 |
| ISSN: | 2374-7951 |
| DOI: | 10.1021/acscentsci.8b00933 |
| Online-Zugang: | Verlag, Volltext: https://doi.org/10.1021/acscentsci.8b00933
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| Verfasserangaben: | Matteo Guerra, Dario Frey, Matthias Hagner, Susanne Dittrich, Michelle Paulsen, Marcus A. Mall and Carsten Schultz |
| Zusammenfassung: | Muco-obstructive lung diseases feature extensive bronchiectasis due to the uncontrolled release of neutrophil serine proteases into the airways. To assess if cathepsin G (CG) is a novel key player in chronic lung inflammation, we developed membrane-bound (mSAM) and soluble (sSAM) FRET reporters. The probes quantitatively revealed elevated CG activity in samples from 46 patients. For future basic science and personalized clinical applications, we developed a rapid, highly informative, and easily translatable small-molecule FRET flow cytometry assay for monitoring protease activity including cathepsin G. We demonstrated that mSAM distinguished healthy from patient cells by FRET-based flow cytometry with excellent correlation to confocal microscopy data. |
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| Beschreibung: | Gesehen am 27.05.2019 |
| Beschreibung: | Online Resource |
| ISSN: | 2374-7951 |
| DOI: | 10.1021/acscentsci.8b00933 |