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Proteomic profiling of mammalian COPII and COPI vesicles

Summary - Intracellular transport and homeostasis of the endomembrane system in eukaryotic cells depend on the formation and fusion of vesicular carriers. Coat protein complex (COP) II vesicles export newly synthesized secretory proteins from the endoplasmic reticulum (ER), whereas COPI vesicles fac...

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Bibliographische Detailangaben
Hauptverfasser: Adolf, Frank (VerfasserIn) , Rhiel, Manuel (VerfasserIn) , Hellwig, Andrea (VerfasserIn) , Béthune, Julien (VerfasserIn) , Wieland, Felix T. (VerfasserIn) , Gao, Qi (VerfasserIn)
Dokumenttyp: Article (Journal)
Sprache:Englisch
Veröffentlicht: January 2, 2019
In: Cell reports
Year: 2019, Jahrgang: 26, Heft: 1, Pages: 250-266
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.12.041
Online-Zugang:Verlag, Volltext: https://doi.org/10.1016/j.celrep.2018.12.041
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S2211124718319715
Volltext
Verfasserangaben:Frank Adolf, Manuel Rhiel, Bernd Hessling, Qi Gao, Andrea Hellwig, Julien Béthune, and Felix T. Wieland
Beschreibung
Zusammenfassung:Summary - Intracellular transport and homeostasis of the endomembrane system in eukaryotic cells depend on the formation and fusion of vesicular carriers. Coat protein complex (COP) II vesicles export newly synthesized secretory proteins from the endoplasmic reticulum (ER), whereas COPI vesicles facilitate traffic from the Golgi to the ER and intra-Golgi transport. Mammalian cells express various isoforms of COPII and COPI coat proteins. To investigate the roles of coat protein paralogs, we have combined in vitro vesicle reconstitution from semi-intact cells with SILAC-based mass spectrometric analysis. Here, we describe the core proteomes of mammalian COPII and COPI vesicles. Whereas the compositions of COPII vesicles reconstituted with various isoforms of the cargo-binding subunit Sec24 differ depending on the paralog used, all of the isoforms of the COPI coat produce COPI-coated vesicles with strikingly similar protein compositions.
Beschreibung:Gesehen am 07.06.2019
Beschreibung:Online Resource
ISSN:2211-1247
DOI:10.1016/j.celrep.2018.12.041

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