Donor desmopressin treatment does not affect transplant outcome in the Fischer to Lewis rat renal transplant model
Objectives: Retrospective studies suggest that donor desmopressin (DDAVP) treatment improves renal transplant outcome. The present study tests the hypothesis that desmopressin neutralizes the graft's endothelium from proinflammatory angiopoietin 2 containing Weibel-Palade bodies in the donor, r...
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| Main Authors: | , , , , , , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
June 2016
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| In: |
Experimental and clinical transplantation
Year: 2016, Volume: 14, Issue: 3, Pages: 299-306 |
| ISSN: | 1304-0855 |
| DOI: | 10.6002/ect.2015.0139 |
| Online Access: | Verlag, kostenfrei, Volltext: http://dx.doi.org/10.6002/ect.2015.0139 Verlag, kostenfrei, Volltext: http://ectrx.org/forms/ectrxcontentshow.php?doi_id=10.6002/ect.2015.0139 
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| Author Notes: | Heiko M. Mundt, Simone Höger, Rüdiger Waldherr, Peter Schnuelle, Bernhard K. Krämer, Benito A. Yard, Uwe Göttmann, Urs Benck |
| Summary: | Objectives: Retrospective studies suggest that donor desmopressin (DDAVP) treatment improves renal transplant outcome. The present study tests the hypothesis that desmopressin neutralizes the graft's endothelium from proinflammatory angiopoietin 2 containing Weibel-Palade bodies in the donor, resulting in reduced Weibel-Palade body release at the time of reperfusion in the recipient. Materials and Methods: Using rat models, we examined the influence of desmopressin treatment on the expression of vasopressin 2 receptors and adhesion molecules in brain-dead donors, with renal function examined in allogeneic recipients. The influence of desmopressin on the expression of adhesion molecules also was tested in vitro. Results: Vasopressin 2 receptors were restricted to collecting ducts and distal tubules and only scarcely found in the renal vasculature. Vasopressin 2 receptor expression was down-regulated in brain-dead rats by desmopressin. Renal expression of vascular cellular adhesion molecule 1 and intercellular adhesion molecule 1 were significantly reduced in these rats. In contrast, angiopoietin 2 did not influence the expression of adhesion molecules in in vitro cultured endothelial cells after tumor necrosis factor α stimulation. Donor desmopressin treatment improved neither renal function nor histology in allogeneic renal transplant recipients. Conclusions: Our data do not support the hypothesis that the clinically observed salutary effect of desmopressin is mediated by depletion of Weibel-Palade bodies in renal allografts. |
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| Item Description: | Gesehen am 24.06.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1304-0855 |
| DOI: | 10.6002/ect.2015.0139 |