Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6
Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their bindin...
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| Main Authors: | , , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
2019
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| In: |
Cell & tissue research
Year: 2018, Volume: 375, Issue: 3, Pages: 665-683 |
| ISSN: | 1432-0878 |
| DOI: | 10.1007/s00441-018-2931-x |
| Online Access: | Verlag, Volltext: https://doi.org/10.1007/s00441-018-2931-x
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| Author Notes: | Gabriel García Caballero, Sebastian Schmidt, Martina Schnölzer, Ursula Schlötzer-Schrehardt, Clemens Knospe, Anna-Kristin Ludwig, Joachim C. Manning, Paul Muschler, Herbert Kaltner, Jürgen Kopitz, Hans-Joachim Gabius |
| Summary: | Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the profile of localization. Having identified the chicken galectin-related interfiber protein (C-GRIFIN) as lens-specific protein present in the main body of adult lens, we here report its interaction with lens proteins in ligand blotting. The assumption for pairing with α-, β- and δ-crystallins was ascertained by mass spectrometric detection of their presence in eluted fractions obtained by affinity chromatography. Biochemical and immunohistochemical monitoring revealed protein presence from about 3-day-old embryos onwards, mostly in the cytoplasm of elongated posterior cells, later in secondary lens fiber cells. On the level of gene expression, its promoter was activated by transcription factor L-Maf alone and together with Pax6 like a crystallin gene, substantiating C-GRIFIN’s status as lens-specific galectin. Using this combined strategy for counterreceptor and expression profiling by bio- and histochemical methods including light, electron and fluorescence microscopy, respective monitoring in lens development can now be taken to the level of the complete galectin family. |
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| Item Description: | First online: 17 October 2018 Gesehen am 28.06.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1432-0878 |
| DOI: | 10.1007/s00441-018-2931-x |