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Leda-1/Pianp is targeted to the basolateral plasma membrane by a distinct intracellular juxtamembrane region and modulates barrier properties and E-Cadherin processing

Leda-1/Pianp is a type-I transmembrane protein which is sorted to the basolateral membrane domain of polarized epithelial cells. Here, we investigated trafficking mechanisms and functions of Leda-1/Pianp in MDCK and MCF-7 cells. Basolateral sorting and posttranslational modifications depended on the...

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Bibliographic Details
Main Authors: Evdokimov, Konstantin (Author) , Schledzewski, Kai (Author) , Winkler, Manuel (Author) , Schneider, Stefan W. (Author) , Goerdt, Sergij (Author) , Géraud, Cyrill (Author)
Format: Article (Journal)
Language:English
Published: 20 May 2016
In: Biochemical and biophysical research communications
Year: 2016, Volume: 475, Issue: 4, Pages: 342-349
ISSN:1090-2104
DOI:10.1016/j.bbrc.2016.05.092
Online Access:Verlag, Volltext: https://doi.org/10.1016/j.bbrc.2016.05.092
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0006291X16308002
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Author Notes:Konstantin Evdokimov, Siladitta Biswas, Kai Schledzewski, Manuel Winkler, Christian Gorzelanny, Stefan W. Schneider, Sergij Goerdt, Cyrill Géraud
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Summary:Leda-1/Pianp is a type-I transmembrane protein which is sorted to the basolateral membrane domain of polarized epithelial cells. Here, we investigated trafficking mechanisms and functions of Leda-1/Pianp in MDCK and MCF-7 cells. Basolateral sorting and posttranslational modifications depended on the intracellular juxtamembrane region. Functionally, Leda-1/Pianp increased the transepithelial electrical resistance generated by a polarized cell sheet. Furthermore, resistance to junctional destabilization by tumor cells was enhanced by Leda-1/Pianp indicating increased stability and tightness of intercellular junctions. While Claudin 1 and 4 expression and activities of small GTPases were not affected, γ-Secretase-mediated cleavage of E-Cadherin was attenuated by Leda-1/Pianp. Regulation of proteolytic processing is thus a molecular mechanism by which Leda-1/Pianp can affect junctional integrity and function.
Item Description:Gesehen am 05.07.2019
Physical Description:Online Resource
ISSN:1090-2104
DOI:10.1016/j.bbrc.2016.05.092

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