Cover Image

Fluorescently labeled cyclodextrin derivatives as exogenous markers for real-time transcutaneous measurement of renal function

Evaluation of renal function is crucial for a number of clinical situations. Here, we reported a novel exogenous fluorescent marker (FITC-HPβCD) to real-time assess renal function by using a transcutaneous fluorescent detection technique. FITC-HPβCD was designed based on the principle of renal clear...

Full description

Saved in:
Bibliographic Details
Main Authors: Huang, Jiaguo (Author) , Weinfurter, Stefanie (Author) , Pretze, Marc (Author) , Kränzlin, Bettina (Author) , Pill, Johannes (Author) , Gretz, Norbert (Author)
Format: Article (Journal)
Language:English
Published: September 9, 2016
In: Bioconjugate chemistry
Year: 2016, Volume: 27, Issue: 10, Pages: 2513-2526
ISSN:1520-4812
DOI:10.1021/acs.bioconjchem.6b00452
Online Access:Verlag, Volltext: https://doi.org/10.1021/acs.bioconjchem.6b00452
Get full text
Author Notes:Jiaguo Huang, Stefanie Weinfurter, Pedro Caetano Pinto, Marc Pretze, Bettina Kränzlin, Johannes Pill, Rodeghiero Federica, Rossana Perciaccante, Leopoldo Della Ciana, Rosalinde Masereeuw, and Norbert Gretz
Description
Summary:Evaluation of renal function is crucial for a number of clinical situations. Here, we reported a novel exogenous fluorescent marker (FITC-HPβCD) to real-time assess renal function by using a transcutaneous fluorescent detection technique. FITC-HPβCD was designed based on the principle of renal clearance of designed drugs. It displays favorable fluorescent properties, high hydrophilicity, low plasma protein binding, and high stability in porcine liver esterase as well as in plasma and nontoxicity. More importantly, FITC-HPβCD can be efficiently and rapidly filtered by glomerulus and completely excreted into urine without proximal tubular reabsorption or secretion in rat models. Additionally, the marker was well-tolerated, with nearly 100% urinary recovery of the given doses, and no metabolism were found. Relying on this novel kidney function marker and transcutaneous devices, we demonstrate a rapid, robust, and convenient approach for real-time assessing renal function without the need of time-consuming blood and urine sample preparation. Our work provides a promising tool for noninvasive real-time monitoring of renal function in vivo.
Item Description:Gesehen am 09.07.2019
Physical Description:Online Resource
ISSN:1520-4812
DOI:10.1021/acs.bioconjchem.6b00452

Similar Items