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Phenotypic and functional characterization of neutrophils and monocytes from patients with myelodysplastic syndrome by flow cytometry

Myelodysplastic syndrome (MDS) is a clonal stem cell disorder frequently associated with inefficient granulopoiesis showing dysplastic polymorphonuclear neutrophils (PMNs). To assess PMN functionality in MDS in a clinical routine setting, 30 MDS patients and ten healthy volunteers were analyzed for...

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Bibliographic Details
Main Authors: Schmidt, Cornelia Sabine (Author) , Nolte, Florian (Author)
Format: Article (Journal)
Language:English
Published: October 2016
In: Cellular immunology
Year: 2016, Volume: 308, Pages: 19-26
ISSN:1090-2163
DOI:10.1016/j.cellimm.2016.07.005
Online Access:Verlag, Volltext: https://doi.org/10.1016/j.cellimm.2016.07.005
Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S0008874916300545
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Author Notes:Cornelia S. Schmidt, Pamela Aranda Lopez, Jörn F. Dopheide, Frank Schmidt, Matthias Theobald, Hansjörg Schild, Evi Lauinger-Lörsch, Florian Nolte, Markus P. Radsak
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Summary:Myelodysplastic syndrome (MDS) is a clonal stem cell disorder frequently associated with inefficient granulopoiesis showing dysplastic polymorphonuclear neutrophils (PMNs). To assess PMN functionality in MDS in a clinical routine setting, 30 MDS patients and ten healthy volunteers were analyzed for PMN and monocyte phenotype and function (degranulation, CD62L shedding, oxidative burst and phagocytosis) upon stimulation with lipopolysaccharide by multi-color flow cytometry (MCFC). Our data show a heterogeneous pattern for CD66, CD16 and CD64 expression on PMNs of MDS patients. CD62L shedding rate and CD66 degranulation were reduced. Interestingly, we detected correlations between the WHO adapted prognostic scoring system (WPSS) and CD16 expression on PMNs as well as the international prognostic scoring system (IPSS) and CD11b degranulation by MCFC, suggesting clinical relevance of MCFC based function testing. In conclusion, MCFC of myelodysplastic immunophenotypes and PMN functionality are applicable in clinical settings, but further prospective studies are needed to assess the practical clinical value of such analyses.
Item Description:Gesehen am 17.07.2019
Available online 7 July 2016
Physical Description:Online Resource
ISSN:1090-2163
DOI:10.1016/j.cellimm.2016.07.005

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