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A fear memory engram and its plasticity in the hypothalamic oxytocin system

Oxytocin (OT) release by axonal terminals onto the central nucleus of the amygdala exerts anxiolysis. To investigate which subpopulation of OT neurons contributes to this effect, we developed a novel method: virus-delivered genetic activity-induced tagging of cell ensembles (vGATE). With the vGATE m...

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Main Authors: Hasan, Mazahir (Author) , Althammer, Ferdinand (Author) , Silva da Gouveia, Miriam (Author) , Goyon, Stephanie (Author) , Eliava, Marina (Author) , Lefevre, Arthur (Author) , Kerspern, Damien (Author) , Schimmer, Jonas (Author) , Raftogianni, Androniki (Author) , Wahis, Jerome (Author) , Knobloch-Bollmann, Hanna Sophie (Author) , Tang, Yan (Author) , Liu, Xinying (Author) , Jain, Apar (Author) , Chavant, Virginie (Author) , Goumon, Yannick (Author) , Weislogel, Jan-Marek (Author) , Hurlemann, René (Author) , Herpertz, Sabine (Author) , Pitzer, Claudia (Author) , Darbon, Pascal (Author) , Dogbevia, Godwin K. (Author) , Bertocchi, Ilaria (Author) , Larkum, Matthew E. (Author) , Sprengel, Rolf (Author) , Bading, Hilmar (Author) , Charlet, Alexandre (Author) , Grinevich, Valéry (Author)
Format: Article (Journal)
Language:English
Published: 16 May 2019
In: Neuron
Year: 2019, Volume: 103, Issue: 1, Pages: 133-146
ISSN:1097-4199
DOI:10.1016/j.neuron.2019.04.029
Online Access:Verlag, kostenfrei, Volltext: http://dx.doi.org/10.1016/j.neuron.2019.04.029
Verlag, kostenfrei, Volltext: http://www.sciencedirect.com/science/article/pii/S0896627319303861
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Author Notes:Mazahir T. Hasan, Ferdinand Althammer, Miriam Silva da Gouveia, Stephanie Goyon, Marina Eliava, Arthur Lefevre, Damien Kerspern, Jonas Schimmer, Androniki Raftogianni, Jerome Wahis, H. Sophie Knobloch-Bollmann, Yan Tang, Xinying Liu, Apar Jain, Virginie Chavant, Yannick Goumon, Jan-Marek Weislogel, René Hurlemann, Sabine C. Herpertz, Claudia Pitzer, Pascal Darbon, Godwin K. Dogbevia, Ilaria Bertocchi, Matthew E. Larkum, Rolf Sprengel, Hilmar Bading, Alexandre Charlet, Valery Grinevich
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Summary:Oxytocin (OT) release by axonal terminals onto the central nucleus of the amygdala exerts anxiolysis. To investigate which subpopulation of OT neurons contributes to this effect, we developed a novel method: virus-delivered genetic activity-induced tagging of cell ensembles (vGATE). With the vGATE method, we identified and permanently tagged a small subpopulation of OT cells, which, by optogenetic stimulation, strongly attenuated contextual fear-induced freezing, and pharmacogenetic silencing of tagged OT neurons impaired context-specific fear extinction, demonstrating that the tagged OT neurons are sufficient and necessary, respectively, to control contextual fear. Intriguingly, OT cell terminals of fear-experienced rats displayed enhanced glutamate release in the amygdala. Furthermore, rats exposed to another round of fear conditioning displayed 5-fold more activated magnocellular OT neurons in a novel environment than a familiar one, possibly for a generalized fear response. Thus, our results provide first evidence that hypothalamic OT neurons represent a fear memory engram.
Item Description:Gesehen am 01.08.2019
Physical Description:Online Resource
ISSN:1097-4199
DOI:10.1016/j.neuron.2019.04.029

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