Signatures of T and B cell development, functional responses and PD-1 upregulation after HCMV latent infections and reactivations in Nod.Rag.Gamma mice humanized with cord blood CD34+ cells
Human cytomegalovirus (HCMV) latency is typically harmless but reactivation can be largely detrimental to immune compromised hosts. We modelled latency and reactivation using a traceable HCMV laboratory strain expressing the Gaussia luciferase reporter gene (HCMV/GLuc) in order to interrogate the vi...
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| Main Authors: | , |
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| Format: | Article (Journal) |
| Language: | English |
| Published: |
22 November 2018
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| In: |
Frontiers in immunology
Year: 2018, Volume: 9 |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2018.02734 |
| Online Access: | Verlag, Volltext: https://doi.org/10.3389/fimmu.2018.02734 Verlag, Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2018.02734/full 
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| Author Notes: | Sebastian J. Theobald, Sahamoddin Khailaie, Michael Meyer-Hermann, Valery Volk, Henning Olbrich, Simon Danisch, Laura Gerasch, Andreas Schneider, Christian Sinzger, Dirk Schaudien, Stefan Lienenklaus, Peggy Riese, Carlos A. Guzman, Constanca Figueiredo, Constantin von Kaisenberg, Loukia M. Spineli, Stephanie Glaesener, Almut Meyer-Bahlburg, Arnold Ganser, Michael Schmitt, Michael Mach, Martin Messerle, and Renata Stripecke |
| Summary: | Human cytomegalovirus (HCMV) latency is typically harmless but reactivation can be largely detrimental to immune compromised hosts. We modelled latency and reactivation using a traceable HCMV laboratory strain expressing the Gaussia luciferase reporter gene (HCMV/GLuc) in order to interrogate the viral modulatory effects on the human adaptive immunity. Humanized mice with long-term (more than seventeen weeks) steady human T and B cell immune reconstitutions were infected with HCMV/GLuc and seven weeks later were further treated with granulocyte-colony stimulating factor (G-CSF) to induce viral reactivations. Whole body bio-luminescence imaging analyses clearly differentiated mice with latent viral infections versus reactivations. Foci of vigorous viral reactivations were detectable in liver, lymph nodes and salivary glands. The number of viral genome copies in various tissues increased upon reactivations and were detectable in sorted human CD14+, CD169+ and CD34+ cells. Compared with non-infected controls, mice after infections and reactivations showed higher thymopoiesis, systemic expansion of Th, CTL, Treg and Tfh cells and functional antiviral T cell responses. Latent infections promoted vast development of memory CD4+ T cells while reactivations triggered a shift towards effector T cells expressing PD-1. Further, reactivations prompted a marked development of B cells, maturation of IgG+ plasma cells and HCMV-specific antibody responses. Multivariate statistical methods were employed using T and B cell immune phenotypic profiles obtained with cells from several tissues of individual mice. The data was used to identify combinations of markers that could predict an HCMV infection versus reactivation status. In spleen, but not in lymph nodes, higher frequencies of effector CD4+ T cells expressing PD-1 were among the factors most suited to distinguish HCMV reactivations from infections. These results suggest a shift from a T cell dominated immune response during latent infections towards an exhausted T cell phenotype and active humoral immune response upon reactivations. In sum, this novel in vivo humanized model combined with advanced analyses highlights a dynamic system clearly specifying the immunological spatial signatures of HCMV latency and reactivations. These signatures can be merged as predictive biomarker clusters that can be applied in the clinical translation of new therapies for the control of HCMV reactivation. |
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| Item Description: | Gesehen am 05.08.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1664-3224 |
| DOI: | 10.3389/fimmu.2018.02734 |