Electrochemical effects after transarterial chemoembolization in combination with percutaneous irreversible electroporation: observations in an acute porcine liver model
Purpose - To evaluate the effects of combined use of transarterial chemoembolization and irreversible electroporation (IRE) for focal tissue ablation in an acute porcine liver model. - Materials and Methods - Two established interventional techniques were combined: IRE with zones of irreversible and...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article (Journal) |
| Language: | English |
| Published: |
18 April 2016
|
| In: |
Journal of vascular and interventional radiology
Year: 2016, Volume: 27, Issue: 6, Pages: 913-921.e2 |
| ISSN: | 1535-7732 |
| DOI: | 10.1016/j.jvir.2016.02.001 |
| Online Access: | Verlag, Volltext: https://doi.org/10.1016/j.jvir.2016.02.001 Verlag, Volltext: http://www.sciencedirect.com/science/article/pii/S1051044316002049 
|
| Author Notes: | Dominik Vollherbst, MD, Robert C. Bertheau, MD, Stefan Fritz, MD, Carolin Mogler, MD, Hans-Ulrich Kauczor, MD, Eduard Ryschich, MD, Boris A. Radeleff, MD, Philippe L. Pereira, MD, and Christof M. Sommer, MD |
| Summary: | Purpose - To evaluate the effects of combined use of transarterial chemoembolization and irreversible electroporation (IRE) for focal tissue ablation in an acute porcine liver model. - Materials and Methods - Two established interventional techniques were combined: IRE with zones of irreversible and reversible electroporation and chemoembolization with microspheres, iodized oil, and doxorubicin. IRE was performed before chemoembolization in two pigs (pigs 1 and 2; IRE/chemoembolization group), chemoembolization was performed before IRE in two pigs (pigs 3 and 4; chemoembolization/IRE group), and only IRE was performed in two pigs (pigs 5 and 6). Five study groups were defined: IRE/chemoembolization (pigs 1 and 2), chemoembolization/IRE (pigs 3 and 4), IRE only (pigs 5 and 6), chemoembolization only (tissue outside the IRE zones in pigs 1-4), and control (untreated liver tissue outside the IRE zones in pigs 5 and 6). Animals were euthanized 2 hours after intervention. Size and shape of IRE zones on contrast-enhanced computed tomography, cell death on light microscopy, and doxorubicin tissue concentrations on chromatography and fluorescence microscopy were analyzed. - Results - Size and shape of IRE zones were not significantly different (eg, P = .067 for volume). A histologic marker for irreversible cell death was positive in IRE/chemoembolization, chemoembolization/IRE, and IRE groups only in the macroscopically visible IRE zones. Doxorubicin tissue concentrations were not significantly different (P = .873). However, in the reversible electroporation (RE) zones, broad areas with intense intranuclear doxorubicin accumulation were observed in IRE/chemoembolization but not in chemoembolization/IRE and chemoembolization groups. - Conclusions - IRE before chemoembolization enhances the intranuclear accumulation of doxorubicin in the RE zone. |
|---|---|
| Item Description: | Gesehen am 09.08.2019 |
| Physical Description: | Online Resource |
| ISSN: | 1535-7732 |
| DOI: | 10.1016/j.jvir.2016.02.001 |