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V-ATPase activity in the TGN/EE is required for exocytosis and recycling in Arabidopsis

In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-A...

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Main Authors: Luo, Yu (Author) , Scholl, Stefan (Author) , Doering, Anett (Author) , Zhang, Yi (Author) , Irani, Niloufer G. (Author) , Di Rubbo, Simone (Author) , Neumetzler, Lutz (Author) , Krishnamoorthy, Praveen (Author) , Van Houtte, Isabelle (Author) , Mylle, Evelien (Author) , Bischoff, Volker (Author) , Vernhettes, Samantha (Author) , Winne, Johan (Author) , Friml, Jiří (Author) , Stierhof, York-Dieter (Author) , Schumacher, Karin (Author) , Persson, Staffan (Author) , Russinova, Eugenia (Author)
Format: Article (Journal)
Language:English
Published: 06 July 2015
In: Nature plants
Year: 2015, Volume: 1, Issue: 7
ISSN:2055-0278
DOI:10.1038/nplants.2015.94
Online Access:Verlag, Volltext: https://doi.org/10.1038/nplants.2015.94
Verlag, Volltext: https://www.nature.com/articles/nplants201594
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Author Notes:Yu Luo, Stefan Scholl, Anett Doering, Yi Zhang, Niloufer G. Irani, Simone Di Rubbo, Lutz Neumetzler, Praveen Krishnamoorthy, Isabelle Van Houtte, Evelien Mylle, Volker Bischoff, Samantha Vernhettes, Johan Winne, Jiří Friml, York-Dieter Stierhof, Karin Schumacher, Staffan Persson and Eugenia Russinova
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Summary:In plants, vacuolar H+-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants.
Item Description:Gesehen am 14.08.2019
Physical Description:Online Resource
ISSN:2055-0278
DOI:10.1038/nplants.2015.94

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